Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine LungAnthos Christofides 1,2, Carol Cao 1,2,3, Rinku Pal 1,2, Halil I. Aksoylar 1,2, Vassiliki A. Boussiotis 1,2
1Division of Hematology-Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, 2Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 3Harvard College
In this study, we present an effective and reproducible protocol to isolate the immune populations of the murine respiratory system. We also provide a method for the identification of all innate and adaptive immune cells that reside in the lungs of healthy mice, using a 9-color-based flow cytometry panel.
