Suny Downstate Medical Center
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
Real-time Iontophoresis with Tetramethylammonium to Quantify Volume Fraction and Tortuosity of Brain Extracellular Space
John Odackal *1, Robert Colbourn *2,3, Namrita Jain Odackal 4, Lian Tao 5, Charles Nicholson 5, Sabina Hrabetova 2
1Department of Medicine, University of Virginia, 2Department of Cell Biology, SUNY Downstate Medical Center, 3Neural and Behavioral Science Graduate Program, SUNY Downstate Medical Center, 4Division of Neonatology, University of Virginia, 5Department of Neuroscience and Physiology, New York University School of Medicine
This protocol describes real-time iontophoresis, a method that measures physical parameters of the extracellular space (ECS) of living brains. The diffusion of an inert molecule released into the ECS is used to calculate the ECS volume fraction and tortuosity. It is ideal for studying acute reversible changes to brain ECS.
Neuroscience
In Vivo Fiber-Coupled Pre-Clinical Confocal Laser-scanning Endomicroscopy (pCLE) of Hippocampal Capillaries in Awake Mice
Rocio Leal-Campanario 1,2, Susana Martinez-Conde 2,3, Stephen L. Macknik 2,3
1División de Neurociencias, Universidad Pablo de Olavide, 2Barrow Neurological Institute, 3Ophthalmology, Neurology, and Physiology/Pharmacology, SUNY Downstate Medical Center
Whereas multiphoton imaging is only effective at limited depths from the tissue’s surface, it is possible to achieve 3 µm resolution imaging at any depth via pCLE. Here, we present a protocol to conduct pCLE imaging to measure microvascular dynamics in the hippocampus of ictal and wild-type mice.
