Hackensack University Medical Center
2 ARTICLES PUBLISHED IN JoVE
Genetics
Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens
Olivier Loudig 1,2,3, Christina Liu 1,2, Thomas Rohan 3, Iddo Z. Ben-Dov 4
1Department of Research, Hackensack University Medical Center, 2Department of Medical Sciences, Seton Hall University, 3Department of Epidemiology and Population Health, Albert Einstein College of Medicine, 4Department of Nephrology and Hypertension, Hadassah - Hebrew University Medical Center
Formalin-fixed paraffin-embedded specimens represent a valuable source of molecular biomarkers of human diseases. Here we present a laboratory-based cDNA library preparation protocol, initially designed with fresh frozen RNA, and optimized for the analysis of archived microRNAs from tissues stored up to 35 years.
Immunology and Infection
A Minimally Invasive, Accurate, and Efficient Technique for Intrathymic Injection in Mice
Michael T. McGuire *1, Andrea Z. Tuckett *2, Faith Myint 2, Johannes L. Zakrzewski 2,3,4
1Department of Radiology, Hackensack University Medical Center, 2Center for Discovery and Innovation, Hackensack Meridian Health, 3Department of Pediatrics, Hackensack University Medical Center, 4Department of Oncology, Georgetown University
The present protocol describes an interventional radiology procedure established for intrathymic injection in mice to avoid the risk of open surgery and improve the accuracy of blind percutaneous injections.
