University of Cardiff

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Using Human Induced Pluripotent Stem Cell-derived Intestinal Organoids to Study and Modify Epithelial Cell Protection Against Salmonella and Other Pathogens

Emily A. Lees^1,2, Jessica L. Forbester^1,3, Sally Forrest², Leanne Kane¹, David Goulding¹, Gordon Dougan^1,2

¹Wellcome Trust Sanger Institute, ²Department of Medicine, University of Cambridge, ³University of Cardiff

Human induced pluripotent stem cell (hiPSC)-derived intestinal organoids offer exciting opportunities to model enteric diseases in vitro. We demonstrate the differentiation of hiPSCs into intestinal organoids (iHOs), the stimulation of these iHOs with cytokines, and the microinjection of Salmonella Typhimurium into the iHO lumen, enabling the study of an epithelial invasion by this pathogen.