Using Human Induced Pluripotent Stem Cell-derived Intestinal Organoids to Study and Modify Epithelial Cell Protection Against Salmonella and Other PathogensEmily A. Lees 1,2, Jessica L. Forbester 1,3, Sally Forrest 2, Leanne Kane 1, David Goulding 1, Gordon Dougan 1,2
1Wellcome Trust Sanger Institute, 2Department of Medicine, University of Cambridge, 3University of Cardiff
Human induced pluripotent stem cell (hiPSC)-derived intestinal organoids offer exciting opportunities to model enteric diseases in vitro. We demonstrate the differentiation of hiPSCs into intestinal organoids (iHOs), the stimulation of these iHOs with cytokines, and the microinjection of Salmonella Typhimurium into the iHO lumen, enabling the study of an epithelial invasion by this pathogen.
