The Forsyth Institute
2 ARTICLES PUBLISHED IN JoVE
Biology
Obtaining Hemocytes from the Hawaiian Bobtail Squid Euprymna scolopes and Observing their Adherence to Symbiotic and Non-Symbiotic Bacteria
Andrew J. Collins 1, Spencer V. Nyholm 1
1Department of Molecular and Cell Biology, University of Connecticut
This video will demonstrate how to obtain hemocytes (blood cells) from the Hawaiian bobtail squid, Euprymna scolopes for use in cell biological and bacterial adhesion assays. Hemocytes will be stained with a fluorescent dye and exposed to GFP-labeled bacteria.
Biology
Establishing Stable Binary Cultures of Symbiotic Saccharibacteria from the Oral Cavity
Andrew J. Collins *1,2, Pallavi P. Murugkar *1,2, Floyd E. Dewhirst 1,2
1The Forsyth Institute, 2Harvard School of Dental Medicine
We demonstrate a method for isolating difficult-to-grow members of the novel bacterial phylum, Saccharibacteria, by filtering dental plaque and co-culturing with host bacteria.
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