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The Forsyth Institute

2 ARTICLES PUBLISHED IN JoVE

Biology

Obtaining Hemocytes from the Hawaiian Bobtail Squid Euprymna scolopes and Observing their Adherence to Symbiotic and Non-Symbiotic Bacteria

Andrew J. Collins¹, Spencer V. Nyholm¹

¹Department of Molecular and Cell Biology, University of Connecticut

This video will demonstrate how to obtain hemocytes (blood cells) from the Hawaiian bobtail squid, Euprymna scolopes for use in cell biological and bacterial adhesion assays. Hemocytes will be stained with a fluorescent dye and exposed to GFP-labeled bacteria.

Biology

Establishing Stable Binary Cultures of Symbiotic Saccharibacteria from the Oral Cavity

Andrew J. Collins^*1,2, Pallavi P. Murugkar^*1,2, Floyd E. Dewhirst^1,2

¹The Forsyth Institute, ²Harvard School of Dental Medicine

We demonstrate a method for isolating difficult-to-grow members of the novel bacterial phylum, Saccharibacteria, by filtering dental plaque and co-culturing with host bacteria.