School of Medicine, University of Pennsylvania
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
Autologous Blood Injection to Model Spontaneous Intracerebral Hemorrhage in Mice
Lauren H. Sansing 1,2,3, Scott E. Kasner 2, Louise McCullough 1, Puneet Agarwal 2, Frank A. Welsh 4, Katalin Kariko 4
1Department of Neurology, University of Connecticut Health Center, 2Department of Neurology, School of Medicine, University of Pennsylvania, 3Department of Neurosurgery, Hartford Hospital, 4Department of Neurosurgery, School of Medicine, University of Pennsylvania
The autologous blood injection model of intracerebral hemorrhage in mice described in this protocol uses the double injection technique to minimize risk of blood reflux up the needle track, no anticoagulants in the pumping system, and eliminates all dead space and expandable tubing in the system.
Neuroscience
Isolation and Culture of Neural Crest Stem Cells from Human Hair Follicles
Ruifeng Yang 1, Xiaowei Xu 1
1Department of Pathology and Laboratory Medicine, School of Medicine, University of Pennsylvania
This article presents a robust protocol for isolation and culture of neural crest stem cells from human hair follicles.
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