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School of Medicine, University of Pennsylvania

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Autologous Blood Injection to Model Spontaneous Intracerebral Hemorrhage in Mice

Lauren H. Sansing^1,2,3, Scott E. Kasner², Louise McCullough¹, Puneet Agarwal², Frank A. Welsh⁴, Katalin Kariko⁴

¹Department of Neurology, University of Connecticut Health Center, ²Department of Neurology, School of Medicine, University of Pennsylvania, ³Department of Neurosurgery, Hartford Hospital, ⁴Department of Neurosurgery, School of Medicine, University of Pennsylvania

The autologous blood injection model of intracerebral hemorrhage in mice described in this protocol uses the double injection technique to minimize risk of blood reflux up the needle track, no anticoagulants in the pumping system, and eliminates all dead space and expandable tubing in the system.

Neuroscience

Isolation and Culture of Neural Crest Stem Cells from Human Hair Follicles

Ruifeng Yang¹, Xiaowei Xu¹

¹Department of Pathology and Laboratory Medicine, School of Medicine, University of Pennsylvania

This article presents a robust protocol for isolation and culture of neural crest stem cells from human hair follicles.