Benemérita Universidad Autónoma de Puebla
2 ARTICLES PUBLISHED IN JoVE
Chemistry
Syntheses, Crystallization, and Spectroscopic Characterization of 3,5-Lutidine N-Oxide Dehydrate
Rosario Merino-García 1, Samuel Hernández-Anzaldo 1, Yasmi Reyes-Ortega 1
1Centro de Química, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla
Herein, we report the synthesis and crystallization of 3,5-lutidine N-oxide dehydrate by a simple protocol that differs from the classical synthesis of pyridine N-oxide. This protocol utilizes different starting material and involves less reaction time to yield a new solvated supramolecular structure, which crystallizes under slow evaporation.
Neuroscience
Label-Free Non-Linear Optics for the Study of Tubulin-Dependent Defects in Central Myelin
Valeria Piazza 1, Milvia Alata 1, Victor H. Hernandez 2, José R. Eguibar 3,4, Carmen Cortes 3
1Centro de Investigaciones en Óptica A.C. (CIO), 2Division of Sciences and Engineering, Department of Chemical, Electronic, and Biomedical Engineering, Universidad de Guanajuato, 3Institute of Physiology, Benemérita Universidad Autónoma de Puebla, 4Dirección General de Desarrollo Internacional, Benemérita Universidad Autónoma de Puebla
In this article, we present a protocol to detect microtubule-loaded oligodendrocytes in a model of tubulinopathy through a simple, innovative second harmonic generation microscopy approach.
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