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Benemérita Universidad Autónoma de Puebla

2 ARTICLES PUBLISHED IN JoVE

Chemistry

Syntheses, Crystallization, and Spectroscopic Characterization of 3,5-Lutidine N-Oxide Dehydrate

Rosario Merino-García¹, Samuel Hernández-Anzaldo¹, Yasmi Reyes-Ortega¹

¹Centro de Química, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla

Herein, we report the synthesis and crystallization of 3,5-lutidine N-oxide dehydrate by a simple protocol that differs from the classical synthesis of pyridine N-oxide. This protocol utilizes different starting material and involves less reaction time to yield a new solvated supramolecular structure, which crystallizes under slow evaporation.

Neuroscience

Label-Free Non-Linear Optics for the Study of Tubulin-Dependent Defects in Central Myelin

Valeria Piazza¹, Milvia Alata¹, Victor H. Hernandez², José R. Eguibar^3,4, Carmen Cortes³

¹Centro de Investigaciones en Óptica A.C. (CIO), ²Division of Sciences and Engineering, Department of Chemical, Electronic, and Biomedical Engineering, Universidad de Guanajuato, ³Institute of Physiology, Benemérita Universidad Autónoma de Puebla, ⁴Dirección General de Desarrollo Internacional, Benemérita Universidad Autónoma de Puebla

In this article, we present a protocol to detect microtubule-loaded oligodendrocytes in a model of tubulinopathy through a simple, innovative second harmonic generation microscopy approach.