Texas Heart Institute
2 ARTICLES PUBLISHED IN JoVE
Bioengineering
Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation
Doris A. Taylor 1, Luiz C. Sampaio 1, Rafael Cabello 1, Abdelmotagaly Elgalad 1, Rohan Parikh 1, R Patrick Wood 2, Kevin A. Myer 2, Alvin T. Yeh 3, Po-Feng Lee 1
1Regenerative Medicine Research, Texas Heart Institute, 2Lifegift Organ Donation Center, 3Biomedical Engineering, Texas A&M University
This method enables decellularization of a complex solid organ using a simple protocol based on osmotic shock and perfusion of ionic detergent with minimal organ matrix disruption. It comprises a novel decellularization technique for human hearts inside a pressurized pouch with real-time monitoring of flow dynamics and cellular debris outflow.
Bioengineering
Generating a Fractal Microstructure of Laminin-111 to Signal to Cells
Camila Hochman-Mendez 1, Tatiana Coelho-Sampaio 2, Ariel J. Kent 3, Jamie L. Inman 3, Mina J. Bissell 3, Claire Robertson 4
1Regenerative Medicine Research, Texas Heart Institute, 2Institute of Biomedical Sciences, Federal University of Rio de Janeiro, 3Biological Systems and Engineering, Lawrence Berkeley National Lab, 4Materials Engineering Division, Lawrence Livermore National Lab
We describe three methods to generate Ln1 polymers with fractal properties that signal to cells differently compared to unpolymerized Ln1.
