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Texas Heart Institute

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation

Doris A. Taylor¹, Luiz C. Sampaio¹, Rafael Cabello¹, Abdelmotagaly Elgalad¹, Rohan Parikh¹, R Patrick Wood², Kevin A. Myer², Alvin T. Yeh³, Po-Feng Lee¹

¹Regenerative Medicine Research, Texas Heart Institute, ²Lifegift Organ Donation Center, ³Biomedical Engineering, Texas A&M University

This method enables decellularization of a complex solid organ using a simple protocol based on osmotic shock and perfusion of ionic detergent with minimal organ matrix disruption. It comprises a novel decellularization technique for human hearts inside a pressurized pouch with real-time monitoring of flow dynamics and cellular debris outflow.

Bioengineering

Generating a Fractal Microstructure of Laminin-111 to Signal to Cells

Camila Hochman-Mendez¹, Tatiana Coelho-Sampaio², Ariel J. Kent³, Jamie L. Inman³, Mina J. Bissell³, Claire Robertson⁴

¹Regenerative Medicine Research, Texas Heart Institute, ²Institute of Biomedical Sciences, Federal University of Rio de Janeiro, ³Biological Systems and Engineering, Lawrence Berkeley National Lab, ⁴Materials Engineering Division, Lawrence Livermore National Lab

We describe three methods to generate Ln1 polymers with fractal properties that signal to cells differently compared to unpolymerized Ln1.