Geneva University Hospitals

3 ARTICLES PUBLISHED IN JoVE

Medicine

Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation

Filippo Molica¹, Séverine Nolli², Pierre Fontana², Brenda Renata Kwak¹

¹Department of Pathology and Immunology, University of Geneva, ²Division of Angiology and Haemostasis & Geneva Platelet Group, Geneva University Hospitals

We describe a straightforward method for the isolation of washed platelets from human blood followed by agonist-induced platelet aggregation measurements by turbidimetry. As an example we apply this method for studying the aggregation response of human platelets to collagen after a pre-incubation with the Pannexin1 channel inhibitor Brilliant Blue FCF.

Medicine

Surgical Training for the Implantation of Neocortical Microelectrode Arrays Using a Formaldehyde-fixed Human Cadaver Model

Pierre Mégevand^1,2, Alain Woodtli¹, Aude Yulzari¹, G. Rees Cosgrove³, Shahan Momjian⁴, Bojan V. Stimec⁵, Marco V. Corniola⁴, Jean H. D. Fasel⁵

¹Wyss Center for Bio and Neuroengineering, Geneva, ²Division of Neurology, Department of Clinical Neuroscience, Geneva University Hospitals, ³Department of Neurosurgery, Brigham and Women's Hospital, Harvard Medical School, ⁴Division of Neurosurgery, Department of Clinical Neuroscience, Geneva University Hospitals, ⁵Clinical Anatomy Research Group, Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva

We designed a procedure in which a formaldehyde-fixed human cadaver is used to assist neurosurgeons in training for the implantation of microelectrode arrays into the neocortex of the human brain.

Bioengineering

Human Neural Organoids for Studying Brain Cancer and Neurodegenerative Diseases

Erika Cosset^*1, Manon Locatelli^*2, Antoine Marteyn², Pierre Lescuyer³, Florence Dall Antonia³, Flavio Maurizio Mor⁴, Olivier Preynat-Seauve⁵, Luc Stoppini⁴, Vannary Tieng²

¹Laboratory of Tumor Immunology, Translational Research Center in Onco-Hematology, Department of Internal Medicine Specialties, Faculty of Medicine, University of Geneva, ²Department of Pathology and Immunology, University Medical Center, University of Geneva, ³Laboratory of Toxicology and Therapeutic Drug Monitoring, Geneva University Hospitals, ⁴Tissue Engineering Laboratory, Hepia/HES-SO, University of Applied Sciences Western Switzerland, ⁵Laboratory of Experimental cell therapy, Department of Diagnostics, Geneva University Hospitals

This study introduces and describes protocols to derive two specific human neural organoids as a relevant and accurate model for studying 1) human glioblastoma development within human neural organoids exclusively in humans and 2) neuron dopaminergic differentiation generating a three-dimensional organoid.