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Geneva University Hospitals

3 ARTICLES PUBLISHED IN JoVE

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Medicine

Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation
Filippo Molica 1, Séverine Nolli 2, Pierre Fontana 2, Brenda Renata Kwak 1
1Department of Pathology and Immunology, University of Geneva, 2Division of Angiology and Haemostasis & Geneva Platelet Group, Geneva University Hospitals

We describe a straightforward method for the isolation of washed platelets from human blood followed by agonist-induced platelet aggregation measurements by turbidimetry. As an example we apply this method for studying the aggregation response of human platelets to collagen after a pre-incubation with the Pannexin1 channel inhibitor Brilliant Blue FCF.

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Medicine

Surgical Training for the Implantation of Neocortical Microelectrode Arrays Using a Formaldehyde-fixed Human Cadaver Model
Pierre Mégevand 1,2, Alain Woodtli 1, Aude Yulzari 1, G. Rees Cosgrove 3, Shahan Momjian 4, Bojan V. Stimec 5, Marco V. Corniola 4, Jean H. D. Fasel 5
1Wyss Center for Bio and Neuroengineering, Geneva, 2Division of Neurology, Department of Clinical Neuroscience, Geneva University Hospitals, 3Department of Neurosurgery, Brigham and Women's Hospital, Harvard Medical School, 4Division of Neurosurgery, Department of Clinical Neuroscience, Geneva University Hospitals, 5Clinical Anatomy Research Group, Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva

We designed a procedure in which a formaldehyde-fixed human cadaver is used to assist neurosurgeons in training for the implantation of microelectrode arrays into the neocortex of the human brain.

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Bioengineering

Human Neural Organoids for Studying Brain Cancer and Neurodegenerative Diseases
Erika Cosset *1, Manon Locatelli *2, Antoine Marteyn 2, Pierre Lescuyer 3, Florence Dall Antonia 3, Flavio Maurizio Mor 4, Olivier Preynat-Seauve 5, Luc Stoppini 4, Vannary Tieng 2
1Laboratory of Tumor Immunology, Translational Research Center in Onco-Hematology, Department of Internal Medicine Specialties, Faculty of Medicine, University of Geneva, 2Department of Pathology and Immunology, University Medical Center, University of Geneva, 3Laboratory of Toxicology and Therapeutic Drug Monitoring, Geneva University Hospitals, 4Tissue Engineering Laboratory, Hepia/HES-SO, University of Applied Sciences Western Switzerland, 5Laboratory of Experimental cell therapy, Department of Diagnostics, Geneva University Hospitals

This study introduces and describes protocols to derive two specific human neural organoids as a relevant and accurate model for studying 1) human glioblastoma development within human neural organoids exclusively in humans and 2) neuron dopaminergic differentiation generating a three-dimensional organoid.

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