The Pirbright Institute
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing
Na Tang 1,2, Yaoyao Zhang 1, Miriam Pedrera 1, Pengxiang Chang 1, Susan Baigent 1, Katy Moffat 1, Zhiqiang Shen 2, Venugopal Nair 1, Yongxiu Yao 1
1The Pirbright Institute & UK-China Centre of Excellence for Research on Avian Diseases, 2Binzhou Animal Science and Veterinary Medicine Academy & UK-China Centre of Excellence for Research on Avian Diseases
Herpesvirus of turkeys (HVT) is widely used as a vector platform for the generation of recombinant vaccines against a number of avian diseases. This article describes a simple and rapid approach for the generation of recombinant HVT-vectored vaccines using an integrated NHEJ-CRISPR/Cas9 and Cre-Lox system.
Immunology and Infection
An Ex Vivo Chicken Primary Bursal-cell Culture Model to Study Infectious Bursal Disease Virus Pathogenesis
Katherine L. Dulwich *1, Amin S. Asfor *1, Alice G. Gray 1, Venugopal Nair 1, Andrew J. Broadbent 1
1The Pirbright Institute
Here we describe the isolation of chicken primary bursal cells from the bursa of Fabricius, the culture and infection of the cells with infectious bursal disease virus, and the quantification of viral replication.
