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The Pirbright Institute

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

Na Tang^1,2, Yaoyao Zhang¹, Miriam Pedrera¹, Pengxiang Chang¹, Susan Baigent¹, Katy Moffat¹, Zhiqiang Shen², Venugopal Nair¹, Yongxiu Yao¹

¹The Pirbright Institute & UK-China Centre of Excellence for Research on Avian Diseases, ²Binzhou Animal Science and Veterinary Medicine Academy & UK-China Centre of Excellence for Research on Avian Diseases

Herpesvirus of turkeys (HVT) is widely used as a vector platform for the generation of recombinant vaccines against a number of avian diseases. This article describes a simple and rapid approach for the generation of recombinant HVT-vectored vaccines using an integrated NHEJ-CRISPR/Cas9 and Cre-Lox system.

Immunology and Infection

An Ex Vivo Chicken Primary Bursal-cell Culture Model to Study Infectious Bursal Disease Virus Pathogenesis

Katherine L. Dulwich^*1, Amin S. Asfor^*1, Alice G. Gray¹, Venugopal Nair¹, Andrew J. Broadbent¹

¹The Pirbright Institute

Here we describe the isolation of chicken primary bursal cells from the bursa of Fabricius, the culture and infection of the cells with infectious bursal disease virus, and the quantification of viral replication.