Taichung Veterans General Hospital
2 ARTICLES PUBLISHED IN JoVE
Bioengineering
Paper-based Devices for Isolation and Characterization of Extracellular Vesicles
Chihchen Chen 1, Bo-Ren Lin 1, Min-Yen Hsu 2, Chao-Min Cheng 1
1Institute of Nanoengineering and Microsystems, National Tsing Hua University, 2Taichung Veterans General Hospital
This protocol details a method to isolate extracellular vesicles (EVs), small membranous particles released from cells, from as little as 10 μl serum samples. This approach circumvents the need for ultracentrifugation, requires only a few minutes of assay time, and enables the isolation of EVs from samples of limited volumes.
Bioengineering
Fabricating Cotton Analytical Devices
Shang-Chi Lin *1, Min-Yen Hsu *2, Chen-Meng Kuan 1, Fan-Gang Tseng 3, Chao-Min Cheng 4
1Institute of Nanoengineering and Microsystems, National Tsing Hua University, 2Department of Ophthalmology, Taichung Veterans General Hospital, 3Department of Engineering and System Science, National Tsing Hua University, 4Institute of Biomedical Engineering, National Tsing Hua University
To investigate simple fabrication approaches for multiple assay needs, we created a fluid-absorbing channel system made of cotton material. This device was used to establish a multiple detection platform, and solve contamination issues that commonly affect lateral flow-based biomedical devices, for clinical urinalysis of nitrite, total protein, and urobilinogen.
