Accedi

Taichung Veterans General Hospital

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Paper-based Devices for Isolation and Characterization of Extracellular Vesicles

Chihchen Chen¹, Bo-Ren Lin¹, Min-Yen Hsu², Chao-Min Cheng¹

¹Institute of Nanoengineering and Microsystems, National Tsing Hua University, ²Taichung Veterans General Hospital

This protocol details a method to isolate extracellular vesicles (EVs), small membranous particles released from cells, from as little as 10 μl serum samples. This approach circumvents the need for ultracentrifugation, requires only a few minutes of assay time, and enables the isolation of EVs from samples of limited volumes.

Bioengineering

Fabricating Cotton Analytical Devices

Shang-Chi Lin^*1, Min-Yen Hsu^*2, Chen-Meng Kuan¹, Fan-Gang Tseng³, Chao-Min Cheng⁴

¹Institute of Nanoengineering and Microsystems, National Tsing Hua University, ²Department of Ophthalmology, Taichung Veterans General Hospital, ³Department of Engineering and System Science, National Tsing Hua University, ⁴Institute of Biomedical Engineering, National Tsing Hua University

To investigate simple fabrication approaches for multiple assay needs, we created a fluid-absorbing channel system made of cotton material. This device was used to establish a multiple detection platform, and solve contamination issues that commonly affect lateral flow-based biomedical devices, for clinical urinalysis of nitrite, total protein, and urobilinogen.