Name: fabricating cotton analytical devices
Uploaded: 2016-08-30T13:00:00.000+00:00
Duration: 5 min 40 s
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这项工作是由科学技术的台湾省补助的部分资助（MOST 104-2628-E-007-001-MY3（CMC）），台中荣民总医院<span style="color: rgb(34, 34, 34); font-family: arial, sans-serif; font-size: 12.8px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 1; word-spacing: 0px; -webkit-text-stroke-width: 0px; display: inline !important; float: none; background-color: rgb(255, 255, 255);">（TCVGH-1056904C（MYH））。

注意:需要正确的实验室卫生习惯。手套和通用的注意事项使用此POC设备时需要。如果适当的消毒程序不能正确执行，可能会出现的结果或感染的污染。 1.准备试纸条设备<ol><li>确定由接触角测定8（ 图3）的清洗棉的外层的疏水性。 </li><li>通过切割清洗棉成5.5厘米×1厘米的片用切纸机（ 图2A）制造的基于棉的分析设备。 </li><li>钻孔（直径:= 0.5厘米），在三排层叠膜的标准用纸;两个后续的孔之间的距离为1厘米。 注:多达36个单独测试条具有三个测试区域每一个都可以使用层叠膜的标准片（5.5厘米×2厘米）（ 图2B）进行。 </li><li>三明治清洁棉（characteriz由层压薄膜， 即 ，叠层膜的一侧的钻孔的片，以及层叠膜的另一侧的未钻孔片材（无孔）的两片外疏水性和内部亲水性）编放置组装成层压包装设备。这增强了设备的机械强度，并且提供了一个外部的，不可渗透层。 注意:通孔的直径从0.5cm到〜层压后0.47厘米收缩。一些轻微的间隙将保持清洁棉花和围绕每个孔的周边层压薄膜之间。这些微小间隙促进在后续步骤中制造的测试垫圈的位置。 </li><li>使用一对剪刀的从组件， 即切个别5.5厘米×1cm的试片的设备，所钻的层压片/化妆棉/未钻孔层压片"三明治"（ 图2C）。让48个设备总数达到创建标准曲线如后文所述（18设备为亚硝酸盐测定中，15个设备的BSA的测定中，以及对尿胆素原试验15设备）。 </li><li>用锋利的刀或剃刀从每个测试区域的中心（未覆盖层压薄膜）切割的狭缝（作为小流体通道），跨越并通过试验区于纵向元件的亲水层，疏水层，谨慎通过夹持装置（ 图2D）的深度中途切断。 </li><li>通过滑入间隙在层叠（ 图2E），每个孔的周边申请纸检测垫（直径:= 0.5厘米）。 </li></ol> 2.准备尿检标准和指示剂溶液<ol><li>通过溶解在100毫升的去离子（DI）水69.0毫克亚硝酸钠制备10.0毫亚硝酸盐原液。在稀释用去离子水亚硝酸盐原液，创造出一系列的亚硝酸盐浓度标准溶液（2,500 1,250，625，312，156，和78μM）（ 图图4A）。 </li><li>通过将415毫克的BSA在100ml PBS溶液制备60微米的牛血清白蛋白（BSA）原液（10磷酸盐缓冲液，0.0027氯化钾和0.137氯化物酸钠; pH 7.0）中。稀释在去离子水中的BSA原液以创建一系列BSA的浓度标准溶液（30，15，7.5，3.75，和1.875微米）（ 图4B）。 </li><li>通过在100毫升去离子水2.5克尿胆素原溶解制备25克/ L的尿胆素原原液。稀释DI水股票尿胆原的解决方案，创造出一系列尿胆原浓度的标准溶液（7.8，15.6，31.2，62.5，和125毫克/升）（ 图4C）的。 </li><li>制备含有50mM磺胺，330 mM柠檬酸和10mM N-（1-萘基）乙二胺二盐酸盐的亚硝酸盐指示剂溶液。 </li><li>制备含有250mM的柠檬酸盐缓冲液（pH 1.8）和四溴蓝色的在95％乙醇3.3毫溶液的BSA指示剂溶液。 </li><li>制备含0.1 M 4二甲胺甲醛尿胆原指示剂溶液。 </li></ol> 3.准备指示灯垫<ol><li>剪切从色谱纸三个圆形形状的垫或盘（直径= 0.5厘米），并插入他们每个人（一个用于在三测定条每个检测）的在每个测试条的设备来创建解决方案的标准曲线（第4节）。 </li><li>在每个组件的三个层压薄膜孔的，观察层压薄膜，并在切孔的非常边缘棉测试垫之间的微小的间隙。注意，在层叠膜中的孔的直径比所述纸测试焊盘略小。 注:总之，这些特征允许对所述装置的棉测试焊盘部分所施加的纸测试垫使纸测试垫滑的边缘进入所述的超薄间隙， 即 ，层叠膜孔的边缘之下。此保持的纸张检测垫就位。 </li><li>准备亚萨亚硝酸盐该装置的y-部分。 <ol><li>涂覆亚硝酸盐测定垫4微升亚硝酸指示剂溶液（在步骤2.4中制备），并允许它在环境温度下完全干燥的。 </li></ol></li><li>准备检测设备的总蛋白定量部分。 <ol><li>涂层的总蛋白测定垫4微升BSA指示剂溶液（在步骤2.5中制备），并允许它在环境温度下完全干燥。 </li></ol></li><li>准备检测设备的检测Uroblinogen部分。 <ol><li>涂覆uroblinogen测定垫4微升uroblinogen指示符（在步骤2.6中制备），并允许它在环境温度下完全干燥的。 </li></ol></li></ol> 4.创建基于棉分析装置标准曲线<ol><li>对于准备每个标准，沾了制备的器件的吸收端（预装检测指标（见第3.4节））转换成标准的解决方案，持续10秒（吸收量〜200 - 300微升）（ 图2F）。 </li><li>放置基于棉花的设备中的周围环境10分钟以完成比色反应（ 图2F）。重复一式三份每个实验。 </li><li>扫描设备来分析每个测试垫（ 图2G）中发生的色度 ​​的变化。在一个桌面扫描仪和在RGB模式与图像分辨率的600dpi的扫描测试垫的玻璃所得测试垫的地方。 JPEG格式保存每个图像。 </li><li>利用图像分析软件来确定各颜色强度（ 图2H）的计算机上分析扫描的图像。 <ol><li>在http://imagej.nih.gov/ij/下载ImageJ的软件。打开扫描的图像文件。在菜单中选择命令的图像，然后选择"类型"为RGB图像转换为8位灰度。 </li><li>使用"椭圆"工具，在工具栏中选择测试点分析区域。选择"分析"菜单中的COMMANDS，然后选择"测量"来分析颜色强度。记录平均强度的结果。 </li></ol></li><li>建立亚硝酸盐，总蛋白，和尿胆素原试验（ 图2I）的标准曲线。 <ol><li>表明不同浓度的标准亚硝酸盐，总蛋白，和尿胆素原的解决方案，并使用stastical软件的二维坐标系统上的相关的平均强度的结果。 </li><li>由二次模型以及用于通过线性回归模型总蛋白和尿胆素原试验的标准曲线拟合为一个亚硝酸盐测定的标准曲线。 </li></ol></li></ol> 5.确定未知样品浓度来确定的标准曲线值<ol><li>以确定亚硝酸盐，总蛋白，并在未知溶液尿胆素原的浓度，使用该设备作为测定标准溶液的浓度和亚硝酸代替被测强度的结果，总蛋白描述，并且urobilinogen测定到建在步骤2中的模型的公式。 </li></ol>

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The authors declare that they have no competing financial interests. S.C.L., C.M.K., F.G.T., and C.M.C. are inventors of a patent (patent No.: US 8691162 B1) owned by National Tsing Hua University on the basis of the work reported in this manuscript. S.C.L., C.M.K., F.G.T., and C.M.C.have patents pending in Taiwan and China based on the work reported in this manuscript. M.Y.H. declares no potential conflict of interest.

在这个协议中的关键步骤包括确定（有不同的疏水性/亲水性）和滤纸（色谱滤纸或定量滤纸）纯棉材质的适当组合。一个精心策划和执行装置的设计呈现了比色法的最佳性能属性。从我们的比色法测定结果，本文提出的基于棉分析设备演示的巨大潜力为多个疾病检测的平台。 目前大多数横向流动基制品（ 例如 ，妊娠试验）提供不超过一个单一测定functon 14以?...

点护理的发展（POC）是价格实惠，功能强大且易于使用的当务之急是改善全球健康1,2诊断设备。特别是，设备由纤维素基材（ 如纸，丝，棉）提供，因为它们无处不在，承受能力，易用性，稳健性和能力，提供快速的结果3-7的低成本分析有前途的分析平台。 在这里，我们揭示了使用横向流动型格式尿检基于棉的分析装置的发展。这种基于棉分析设备提供了几个关键的优势，另一种检测方法:一）用最少的人的努力制作;二）成本低; ⅲ）将要使用的能力，以进行无交叉污染问题的多个，不同的测定; 。ⅳ）设备无关， 也就是说 ，要无额外的设备和/或电力运行的能力;并且，V）速度（比色分析可在10分钟内完成）。 这种基于棉的分析装置的结构可以分为四个部分:ⅰ）棉这是它的外部疏水层上天然疏水; ⅱ）棉即内部的亲水性和用作液体芯吸的输送通道;三）结合并压缩所用的棉花，但包含里钻出来的反应/测试垫放置孔复合膜;和，ⅳ）色谱纸检测垫，其上涂布/嵌入反应试剂，放置在棉（具体而言，在该空间钻出层叠膜），作为比色分析反应区的外表面上（ 即 ，亚硝酸盐，总蛋白，pH值，和尿胆素原测定）和结果的显示。 试验的基本机理如下。基于棉花分析装置，拿下那些通过部门穿透半路上行棉材料h的创建流动通道，允许样品流体到达所使用的反应垫。分析装置的吸收边缘浸入目标样品，随后溶液是沿从吸收端向测试焊盘（ 图1）的流体通道恶。因为测试垫的吸收强度比棉花时，由测试焊盘吸收溶液被牢固地容纳在测试垫纸内部，使得没有回流返回到流体通道，并且比色结果变成随后固定在测试垫材料。在反应结束时，比色结果经由桌面扫描器记录，并通过图像分析软件进行分析。

<table><tbody><tr><td>bovine serum albumin</td><td>Sigma-Aldrich, US</td><td>No. 9048468</td><td>&amp;ge; 99%</td></tr><tr><td>nitrite&amp;nbsp;</td><td>Sigma-Aldrich, US</td><td>No. 7632000</td><td>&amp;ge; 99%</td></tr><tr><td>urobilinogen&amp;nbsp;</td><td>Santa Cruz Bio, US</td><td>No. SC-296690</td><td></td></tr><tr><td>citrate</td><td>Sigma-Aldrich U.S</td><td>No. 6132043</td><td>&amp;ge; 99%</td></tr><tr><td>tetrabromophenol blue</td><td>Sigma-Aldrich U.S</td><td>No. 4430255</td><td>&amp;ge; 99%</td></tr><tr><td>sulfanilamide</td><td>Sigma-Aldrich U.S</td><td>No. 63741</td><td>&amp;ge; 99%</td></tr><tr><td>citric acid&amp;nbsp;</td><td>Sigma-Aldrich U.S</td><td>No. 77929</td><td>&amp;ge; 99.5%</td></tr><tr><td>&amp;nbsp;N-(1-naphthyl) ethylenediamine dihydrochloride</td><td>Sigma-Aldrich U.S</td><td>No. 1465254</td><td>&amp;ge; 98%</td></tr><tr><td>4-(Dimethylamine)benzaldehyde</td><td>AlfaAesar, U.S</td><td>No. A11712</td><td>&amp;ge; 98%</td></tr><tr><td>Methyl Red sodium salt</td><td>sigma, U.S</td><td>No. 114502</td><td>&amp;ge;95%</td></tr><tr><td>Bromothyle blue</td><td>sigma, U.S</td><td>No. 114413</td><td>&amp;ge;95%</td></tr><tr><td>Shiseido Cleansing Cotton</td><td>Shiseido, Japan</td><td>No. 79014</td><td></td></tr><tr><td>chromatography paper</td><td>GE Healthcare Whatman, Springfield Mill, UK</td><td>No. 30306132</td><td></td></tr><tr><td>plastic substrate</td><td>lamination film, MAS</td><td>A4</td><td>216 mm x 303 mm</td></tr><tr><td>scanner</td><td>microtek scanmaker</td><td>&amp;nbsp;i2400</td><td></td></tr><tr><td>paper cutter</td><td>Life paper cutter</td><td>No.306</td><td></td></tr><tr><td>laminator</td><td>AURORA&amp;nbsp;</td><td>LM4231H</td><td></td></tr><tr><td>laminator film</td><td>UNI LAMI&amp;nbsp;</td><td>4A</td><td></td></tr></tbody></table>

fabricating cotton analytical devices

一个强大的，低成本的分析设备应具有用户友好，快速，而且经济实惠。这样的设备也应该能够拥有稀缺的样品进行操作，并提供后续治疗的信息。在这里，我们证明基于棉花尿检的发展（ 即 ，亚硝酸盐，总蛋白，和尿胆素原测定），它采用一个侧流为基础的格式，并且价格便宜，容易制造，快速，并且可以被用来进行分析装置多个测试无交叉污染的担忧。棉是由与可以被利用为基于流的分析自然吸收性质的纤维素纤维。我们基于棉分析设备的简单而优雅的制作工艺在这项研究中描述。棉花结构和测试垫的配置需要的疏水性和各材料的吸收强度的优势。由于这些物理特性的，比色的结果可以持续粘附在测试垫。该器件使医生能够得到及时的临床信息，并显示出巨大的潜力，作为早期干预的工具。

我们通过使用可商购的清洁棉特征在于亲水性（内部部分）和疏水性（外部部分）的属性（ 图1A）成功地展示了基于棉的分析装置的发展。 图3示出了接触角测量的结果。外棉的疏水界面是127.35°±4.73°。从一个用户友好的角度来看，这里采用比色分析可直接通过肉眼观测，并通过颜色强度进一步定量分析（ 图2F）。尿总蛋白在正常个?...

Watch this Scientific Journal Video about Fabricating Cotton Analytical Devices at JoVE.com

Fabricating Cotton Analytical Devices

To investigate simple fabrication approaches for multiple assay needs, we created a fluid-absorbing channel system made of cotton material. This device was used to establish a multiple detection platform, and solve contamination issues that commonly affect lateral flow-based biomedical devices, for clinical urinalysis of nitrite, total protein, and urobilinogen.

制造棉分析仪器

A robust, low-cost analytical device should be user-friendly, rapid, and affordable. Such devices should also be able to operate with scarce samples and ...

Research

JoVE Journal

Bioengineering

6.7K 次观看.  National Tsing Hua University. To investigate simple fabrication approaches for multiple assay needs, we created a fluid-absorbing channel system made of cotton material. This device was used to establish a multiple detection platform, and solve contamination issues that commonly affect lateral flow-based biomedical devices, for clinical urinalysis of nitrite, total protein, and urobilinogen.

视频: Fabricating Cotton Analytical Devices

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

As medicine is currently practiced, doctors send specimens to a central laboratory for testing and thus must wait hours or days to 
receive the results. Many patients would be better served by rapid, bedside tests. To this end our laboratory and others have developed a versatile, reagentless 
biosensor platform that supports the quantitative, reagentless, electrochemical detection of nucleic acids (DNA, RNA), proteins (including antibodies) and small 
molecules analytes directly in unprocessed clinical and environmental samples. In this video, we demonstrate the preparation and use of several biosensors in this 
"E-DNA" class. In particular, we fabricate and demonstrate sensors for the detection of a target DNA sequence in a polymerase chain reaction mixture, an HIV-specific antibody and the drug cocaine. The preparation procedure requires only three hours of hands-on effort followed by an overnight incubation, and their use requires only minutes.

"E-DNA" sensors, reagentless, electrochemical biosensors that perform well even when challenged directly in blood and other complex matrices, have been adapted to the detection of a wide range of nucleic acid, protein and small molecule analytes. Here we present a general procedure for the fabrication and use of such sensors.

无试剂检测核酸，蛋白质和小分子的电化学DNA生物传感器的制备

As medicine is currently practiced, doctors send specimens to a central laboratory for testing and thus must wait hours or days to 
receive the results. ...

科研

生物工程

Fabrication of Large-area Free-standing Ultrathin Polymer Films

This procedure describes a method for the fabrication of large-area and ultrathin free-standing polymer films. Typically, ultrathin films are prepared using either sacrificial layers, which may damage the film or affect its mechanical properties, or they are made on freshly cleaved mica, a substrate that is difficult to scale. Further, the size of ultrathin film is typically limited to a few square millimeters. In this method, we modify a surface with a polyelectrolyte that alters the strength of adhesion between polymer and deposition substrate. The polyelectrolyte can be shown to remain on the wafer using spectroscopy, and a treated wafer can be used to produce multiple films, indicating that at best minimal amounts of the polyelectrolyte are added to the film. The process has thus far been shown to be limited in scalability only by the size of the coating equipment, and is expected to be readily scalable to industrial processes. In this study, the protocol for making the solutions, preparing the deposition surface, and producing the films is described.

We describe a method for the fabrication of large-area (up to 13 cm diameter) and ultrathin (as thin as 8 nm) polymer films. Instead of using a sacrificial interlayer to delaminate the film from its substrate, we use a self-limiting surface treatment suitable for arbitrarily large areas.

大面积的制造自立性的超薄聚合物薄膜

This procedure describes a method for the fabrication of large-area and ultrathin free-standing polymer films.  Typically, ultrathin films are prepared ...

化学

Fabrication of Carbon Nanotube High-Frequency Nanoelectronic Biosensor for Sensing in High Ionic Strength Solutions

The unique electronic properties and high surface-to-volume ratios of single-walled carbon nanotubes (SWNT) and semiconductor nanowires (NW) 1-4 make them good candidates for high sensitivity biosensors. When a charged molecule binds to such a sensor surface, it alters the carrier density5 in the sensor, resulting in changes in its DC conductance. However, in an ionic solution a charged surface also attracts counter-ions from the solution, forming an electrical double layer (EDL). This EDL effectively screens off the charge, and in physiologically relevant conditions ~100 millimolar (mM), the characteristic charge screening length (Debye length) is less than a nanometer (nm). Thus, in high ionic strength solutions, charge based (DC) detection is fundamentally impeded6-8.
We overcome charge screening effects by detecting molecular dipoles rather than charges at high frequency, by operating carbon nanotube field effect transistors as high frequency mixers9-11. At high frequencies, the AC drive force can no longer overcome the solution drag and the ions in solution do not have sufficient time to form the EDL. Further, frequency mixing technique allows us to operate at frequencies high enough to overcome ionic screening, and yet detect the sensing signals at lower frequencies11-12. Also, the high transconductance of SWNT transistors provides an internal gain for the sensing signal, which obviates the need for external signal amplifier.
Here, we describe the protocol to (a) fabricate SWNT transistors, (b) functionalize biomolecules to the nanotube13, (c) design and stamp a poly-dimethylsiloxane (PDMS) micro-fluidic chamber14 onto the device, and (d) carry out high frequency sensing in different ionic strength solutions11.

We describe the device fabrication and measurement protocol for carbon nanotube based high frequency biosensors. The high frequency sensing technique mitigates the fundamental ionic (Debye) screening effect and allows nanotube biosensor to be operated in high ionic strength solutions where conventional electronic biosensors fail. Our technology provides a unique platform for point-of-care (POC) electronic biosensors operating in physiologically relevant conditions.

制备碳纳米管高频纳电子生物传感器的传感高离子强度的解决方案

The unique electronic properties and high surface-to-volume ratios of single-walled carbon nanotubes (SWNT) and semiconductor nanowires (NW) 1-4 make them ...

Microfluidic Dry-spinning and Characterization of Regenerated Silk Fibroin Fibers

The protocol demonstrates a method for mimicking the spinning process of silkworm. In the native spinning process, the contracting spinning duct enables the silk proteins to be compact and ordered by shearing and elongation forces. Here, a biomimetic microfluidic channel was designed to mimic the specific geometry of the spinning duct of the silkworm. Regenerated silk fibroin (RSF) spinning doped with high concentration, was extruded through the microchannel to dry-spin fibers at ambient temperature and pressure. In the post-treated process, the as-spun fibers were drawn and stored in ethanol aqueous solution. Synchrotron radiation wide-angle X-ray diffraction (SR-WAXD) technology was used to investigate the microstructure of single RSF fibers, which were fixed to a sample holder with the RSF fiber axis normal to the microbeam of the X-ray. The crystallinity, crystallite size, and crystalline orientation of the fiber were calculated from the WAXD data. The diffraction arcs near the equator of the two-dimensional WAXD pattern indicate that the post-treated RSF fiber has a high orientation degree.

A protocol for the microfluidic spinning and microstructure characterization of regenerated silk fibroin monofilament is presented.

微流控干纺及再生丝素纤维的表征

The protocol demonstrates a method for mimicking the spinning process of silkworm. In the native spinning process, the contracting spinning duct enables ...

Fabrication of an Optical Cell Dryer for the Spectroscopic Analysis Cells

Optical cells, which are experimental instruments, are small, square tubes sealed on one side. A sample is placed in this tube, and a measurement is performed with a spectroscope. The materials used for optical cells generally include quartz glass or plastic, but expensive quartz glass is reused by removing substances, other than liquids, to be analyzed that adhere to the interior of the container. In such a case, the optical cells are washed with water or ethanol and dried. Then, the next sample is added and measured. Optical cells are dried naturally or with a manual hairdryer. However, drying takes time, which makes it one of the factors that increase the experiment time. In this study, the objective is to drastically reduce the drying time with a dedicated automatic dryer that can dry multiple optical cells at once. To realize this, a circuit was designed for a microcomputer, and the hardware using it was independently designed and manufactured.

A protocol for fabricating a device for simultaneously drying multiple optical cells is presented.

光谱分析细胞光学细胞干燥机的研制

Optical cells, which are experimental instruments, are small, square tubes sealed on one side. A sample is placed in this tube, and a measurement is ...

工程学

Hybrid Printing for the Fabrication of Smart Sensors

A method to combine additively manufactured substrates or foils and multilayer inkjet printing for the fabrication of sensor devices is presented. First, three substrates (acrylate, ceramics, and copper) are prepared. To determine the resulting material properties of these substrates, profilometer, contact angle, scanning electron microscope (SEM), and focused ion beam (FIB) measurements are done. The achievable printing resolution and suitable drop volume for each substrate are, then, found through the drop size tests. Then, layers of insulating and conductive ink are inkjet printed alternately to fabricate the target sensor structures. After each printing step, the respective layers are individually treated by photonic curing. The parameters used for the curing of each layer are adapted depending on the printed ink, as well as on the surface properties of the respective substrate. To confirm the resulting conductivity and to determine the quality of the printed surface, four-point probe and profilometer measurements are done. Finally, a measurement set-up and results achieved by such an all-printed sensor system are shown to demonstrate the achievable quality.

Here we present a protocol for the fabrication of inkjet-printed multilayer sensor structures on additively manufactured substrates and foil.

用于智能传感器制造的混合打印

A method to combine additively manufactured substrates or foils and multilayer inkjet printing for the fabrication of sensor devices is presented. First, ...

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Paper wicks fluids autonomously due to capillary action. By patterning paper with hydrophobic barriers, the transport of fluids can be controlled and directed within a layer of paper. Moreover, stacking multiple layers of patterned paper creates sophisticated three-dimensional microfluidic networks that can support the development of analytical and bioanalytical assays. Paper-based microfluidic devices are inexpensive, portable, easy to use, and require no external equipment to operate. As a result, they hold great promise as a platform for point-of-care diagnostics. In order to properly evaluate the utility and analytical performance of paper-based devices, suitable methods must be developed to ensure their manufacture is reproducible and at a scale that is appropriate for laboratory settings. In this manuscript, a method to fabricate a general device architecture that can be used for paper-based immunoassays is described. We use a form of additive manufacturing (multi-layer lamination) to prepare devices that comprise multiple layers of patterned paper and patterned adhesive. In addition to demonstrating the proper use of these three-dimensional paper-based microfluidic devices with an immunoassay for human chorionic gonadotropin (hCG), errors in the manufacturing process that may result in device failures are discussed. We expect this approach to manufacturing paper-based devices will find broad utility in the development of analytical applications designed specifically for limited-resource settings.

We detail a method to fabricate three-dimensional paper-based microfluidic devices for use in the development of immunoassays. Our approach to device assembly is a type of multilayer, additive manufacturing. We demonstrate a sandwich immunoassay to provide representative results for these types of paper-based devices.

三维纸基微流体装置的制造用于免疫分析

Paper wicks fluids autonomously due to capillary action. By patterning paper with hydrophobic barriers, the transport of fluids can be controlled and ...

A Simple and Scalable Fabrication Method for Organic Electronic Devices on Textiles

Today, wearable electronics devices combine a large variety of functional, stretchable, and flexible technologies. However, in many cases, these devices cannot be worn under everyday conditions. Therefore, textiles are commonly considered the best substrate to accommodate electronic devices in wearable use. In this paper, we describe how to selectively pattern organic electroactive materials on textiles from a solution in an easy and scalable manner. This versatile deposition technique enables the fabrication of wearable organic electronic devices on clothes.

In this paper, we present a protocol to selectively deposit organic materials on textiles, which allows for the direct integration of organic electronic devices with wearables. The fabricated devices can be fully integrated in textiles, respecting their mechanical appearance and enabling sensing capabilities.

关于纺织品有机电子设备的简单和可扩展的制作方法

Today, wearable electronics devices combine a large variety of functional, stretchable, and flexible technologies. However, in many cases, these devices ...

纳米纤维素纸基分析装置的微压花制备  

Microembossing: A Convenient Process for Fabricating Microchannels on Nanocellulose Paper-Based Microfluidics

Nanopaper, derived from nanofibrillated cellulose, has generated considerable interest as a promising material for microfluidic applications. Its appeal lies in a range of excellent&#160;qualities, including an exceptionally smooth surface, outstanding optical transparency, a uniform nanofiber matrix with nanoscale porosity, and customizable chemical properties. Despite the rapid growth of nanopaper-based microfluidics, the current techniques used to create microchannels on nanopaper, such as 3D printing, spray coating, or manual cutting and assembly, which are crucial for practical applications, still possess certain limitations, notably susceptibility to contamination. Furthermore, these methods are restricted to the production of millimeter-sized channels. This study introduces a straightforward process that utilizes convenient plastic micro-molds for simple microembossing operations to fabricate microchannels on nanopaper, achieving a minimum width of 200 &#181;m. The developed microchannel outperforms existing approaches, achieving a fourfold improvement, and can be fabricated within 45 min. Furthermore, fabrication parameters have been optimized, and a convenient quick-reference table is provided for application developers. The proof-of-concept for a laminar mixer, droplet generator, and functional nanopaper-based analytical devices (NanoPADs) designed for Rhodamine B sensing using surface-enhanced Raman spectroscopy was demonstrated. Notably, the NanoPADs exhibited exceptional performance with improved limits of detection. These outstanding results can be attributed to the superior optical properties of nanopaper and the recently developed accurate microembossing method, enabling the integration and fine-tuning of the NanoPADs.

作者聚焦：通过纳米纸上的微通道制造彻底改变微流体 

This protocol describes a straightforward process that utilizes convenient plastic micro-molds for simple microembossing operations to fabricate microchannels on nanofibrillated cellulose paper, achieving a minimum width of 200 &#181;m.

微压花：在纳米纤维素纸基微流控上制造微通道的便捷工艺

Nanopaper, derived from nanofibrillated cellulose, has generated considerable interest as a promising material for microfluidic applications. Its appeal ...

制造棉分析仪器

摘要

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此视频中的章节

无试剂检测核酸，蛋白质和小分子的电化学DNA生物传感器的制备

大面积的制造自立性的超薄聚合物薄膜

制备碳纳米管高频纳电子生物传感器的传感高离子强度的解决方案

微流控干纺及再生丝素纤维的表征

光谱分析细胞光学细胞干燥机的研制

用于智能传感器制造的混合打印

三维纸基微流体装置的制造用于免疫分析

关于纺织品有机电子设备的简单和可扩展的制作方法

微压花：在纳米纤维素纸基微流控上制造微通道的便捷工艺

微压花：在纳米纤维素纸基微流控上制造微通道的便捷工艺