Shanghai Institute of Technology
3 ARTICLES PUBLISHED IN JoVE
Environment
Using Caenorhabditis elegans for Studying Trans- and Multi-Generational Effects of Toxicants
Zhuo Li 1,2, Fangting Ai 3, Jing Zhang 4, Zhenyang Yu 1,2, Daqiang Yin 1,2
1College of Environmental Sciences and Engineering, Tongji University, 2Shanghai Institute of Pollution Control and Ecological Security, 3Jiaxing Tongji Institute of Environment, 4College of Ecological Technique and Engineering, Shanghai Institute of Technology
Trans- and multi-generational effects of persistent chemicals are essential in judging their long-term consequences in the environment and on the human health. We provide novel detailed methods for studying trans- and multi-generational effects using free-living nematode Caenorhabditis elegans.
Engineering
Measurement of Chladni Mode Shapes with an Optical Lever Method
Rong Feng *1, Yan Luo *1, Yixue Dong 1, Mengke Ma 2, Yuqi Wang 2, Jing Zhang 3, Wenjiang Ma 3, Donghuan Liu 4
1School of Materials Science and Engineering, University of Science and Technology Beijing, 2School of Civil and Resource Engineering, University of Science and Technology Beijing, 3Basic Experimental Center for Natural Science, University of Science and Technology Beijing, 4School of Mathematics and Physics, University of Science and Technology Beijing
A simple method of measuring the Chladni mode shape on an elastic plate by the principle of an optical lever is proposed.
Cancer Research
Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells
Jing Zhang 1, Midory Thorikay 1, Gerard van der Zon 1, Maarten van Dinther 1, Peter ten Dijke 1
1Oncode Institute and Department of Cell Chemical Biology, Leiden University Medical Center
We describe a systematic workflow to investigate TGF-β signaling and TGF-β-induced EMT by studying the protein and gene expression involved in this signaling pathway. The methods include Western blotting, a luciferase reporter assay, qPCR, and immunofluorescence staining.
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