Baker Heart and Diabetes Institute

2 ARTICLES PUBLISHED IN JoVE

Medicine

Body Composition and Metabolic Caging Analysis in High Fat Fed Mice

Graeme I. Lancaster¹, Darren C. Henstridge¹

This protocol describes the use of a body composition analyzer and metabolic animal monitoring system to characterize body composition and metabolic parameters in mice. An obesity model induced by high-fat feeding is used as an example for the application of these techniques.

Biology

A Step-By-Step Method to Detect Neutralizing Antibodies Against AAV using a Colorimetric Cell-Based Assay

Sebastian Bass-Stringer^1,2, Colleen J. Thomas^2,3, Clive N. May³, Paul Gregorevic⁴, Kate L. Weeks^1,5,6, Julie R. McMullen^1,2,5,6,7

¹Baker Heart and Diabetes Institute, ²Department of Physiology, Anatomy and Microbiology, La Trobe University, ³Florey Institute of Neuroscience and Mental Health, University of Melbourne, ⁴Department of Physiology, Centre for Muscle Research (CMR), The University of Melbourne, ⁵Department of Diabetes, Central Clinical School, Monash University, ⁶Baker Department of Cardiometabolic Health, The University of Melbourne, ⁷Department of Physiology and Department of Medicine Alfred Hospital, Monash University

A comprehensive laboratory protocol and analysis workflow are described for a rapid, cost-effective, and straightforward colorimetric cell-based assay to detect neutralizing elements against AAV6.