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Baker Heart and Diabetes Institute

2 ARTICLES PUBLISHED IN JoVE

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Medicine

Body Composition and Metabolic Caging Analysis in High Fat Fed Mice
Graeme I. Lancaster 1, Darren C. Henstridge 1
1Baker Heart and Diabetes Institute

This protocol describes the use of a body composition analyzer and metabolic animal monitoring system to characterize body composition and metabolic parameters in mice. An obesity model induced by high-fat feeding is used as an example for the application of these techniques.

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Biology

A Step-By-Step Method to Detect Neutralizing Antibodies Against AAV using a Colorimetric Cell-Based Assay
Sebastian Bass-Stringer 1,2, Colleen J. Thomas 2,3, Clive N. May 3, Paul Gregorevic 4, Kate L. Weeks 1,5,6, Julie R. McMullen 1,2,5,6,7
1Baker Heart and Diabetes Institute, 2Department of Physiology, Anatomy and Microbiology, La Trobe University, 3Florey Institute of Neuroscience and Mental Health, University of Melbourne, 4Department of Physiology, Centre for Muscle Research (CMR), The University of Melbourne, 5Department of Diabetes, Central Clinical School, Monash University, 6Baker Department of Cardiometabolic Health, The University of Melbourne, 7Department of Physiology and Department of Medicine Alfred Hospital, Monash University

A comprehensive laboratory protocol and analysis workflow are described for a rapid, cost-effective, and straightforward colorimetric cell-based assay to detect neutralizing elements against AAV6.

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