Quantitation to Determine the Neutralizing Activity Using ImageJ
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Results: Transduction Assay and Assessment of Neutralizing Antibodies (Nabs) Against AAV6 in Sheep Samples
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Conclusion
副本
Preexisting antibodies that neutralize AAV pose a barrier to the advancement of AAV gene therapies. This assay is a screening tool to detect neutralizing antibodies against AAV. The main advantage is that it's cost-effective, time efficient, easy
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A comprehensive laboratory protocol and analysis workflow are described for a rapid, cost-effective, and straightforward colorimetric cell-based assay to detect neutralizing elements against AAV6.