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Advanced Cell Diagnostics, Inc.

4 ARTICLES PUBLISHED IN JoVE

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Medicine

RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma
Hongwei Wang 1, Mindy Xiao-Ming Wang 1, Nan Su 1, Li-chong Wang 1, Xingyong Wu 1, Son Bui 1, Allissa Nielsen 1, Hong-Thuy Vo 1, Nina Nguyen 1, Yuling Luo 1, Xiao-Jun Ma 1
1Advanced Cell Diagnostics, Inc.

The presence of high-risk HPV in head and neck tumor tissue is associated with favorable outcomes. The recently developed RNA in situ hybridization technique called RNAscope allows direct visualization of HPV E6/E7 mRNA in FFPE tissue sections.

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Biology

Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay
Courtney M. Anderson 1, Annelies Laeremans 1, Xiao-Ming Mindy Wang 1, Xingyong Wu 1, Bingqing Zhang 1, Emerald Doolittle 1, Jeffrey Kim 1, Na Li 1, Helly Xiao Yan Pimentel 1, Emily Park 1, Xiao-Jun Ma 1
1Advanced Cell Diagnostics, Inc

Here, we describe an in situ hybridization assay which enables sensitive and specific detection of sequences as short as 50 nucleotides with single-nucleotide resolution at the single-cell level. The assay, which can be performed manually or automatically, can enable visualization of splice variants, short sequences, and mutations within the tissue context.

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Medicine

Three-Dimensional Printing Guide Template Assisted Percutaneous Vertebroplasty (PVP)
PeiLun Hu *1, JiSheng Lin *1, JunChuan Xu 1, Hai Meng 1, Nan Su 1, Yong Yang *1, Qi Fei 1
1Department of Orthopedics, Beijing Friendship Hospital, Capital Medical University

Herein, we present a three-dimensional printing guide template for percutaneous vertebraplasty. A patient with a T11 vertebral compression fracture was selected as a case study.

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Immunology and Infection

Isolation of Primary Mouse Lung Endothelial Cells
Erika Wong 1,2, Nina Nguyen 2, Judith Hellman 2
1Department of Pediatrics, Division of Critical Care, UCSF Benioff Children's Hospital, 2Department of Anesthesia and Perioperative Care, University of California, San Francisco

In this article, primary lung endothelial cells were isolated and cultured from neonatal mice.

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