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Advanced Cell Diagnostics, Inc.

4 ARTICLES PUBLISHED IN JoVE

Medicine

RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma

Hongwei Wang¹, Mindy Xiao-Ming Wang¹, Nan Su¹, Li-chong Wang¹, Xingyong Wu¹, Son Bui¹, Allissa Nielsen¹, Hong-Thuy Vo¹, Nina Nguyen¹, Yuling Luo¹, Xiao-Jun Ma¹

¹Advanced Cell Diagnostics, Inc.

The presence of high-risk HPV in head and neck tumor tissue is associated with favorable outcomes. The recently developed RNA in situ hybridization technique called RNAscope allows direct visualization of HPV E6/E7 mRNA in FFPE tissue sections.

Biology

Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay

Courtney M. Anderson¹, Annelies Laeremans¹, Xiao-Ming Mindy Wang¹, Xingyong Wu¹, Bingqing Zhang¹, Emerald Doolittle¹, Jeffrey Kim¹, Na Li¹, Helly Xiao Yan Pimentel¹, Emily Park¹, Xiao-Jun Ma¹

¹Advanced Cell Diagnostics, Inc

Here, we describe an in situ hybridization assay which enables sensitive and specific detection of sequences as short as 50 nucleotides with single-nucleotide resolution at the single-cell level. The assay, which can be performed manually or automatically, can enable visualization of splice variants, short sequences, and mutations within the tissue context.

Medicine

Three-Dimensional Printing Guide Template Assisted Percutaneous Vertebroplasty (PVP)

PeiLun Hu^*1, JiSheng Lin^*1, JunChuan Xu¹, Hai Meng¹, Nan Su¹, Yong Yang^*1, Qi Fei¹

¹Department of Orthopedics, Beijing Friendship Hospital, Capital Medical University

Herein, we present a three-dimensional printing guide template for percutaneous vertebraplasty. A patient with a T11 vertebral compression fracture was selected as a case study.

Immunology and Infection

Isolation of Primary Mouse Lung Endothelial Cells

Erika Wong^1,2, Nina Nguyen², Judith Hellman²

¹Department of Pediatrics, Division of Critical Care, UCSF Benioff Children's Hospital, ²Department of Anesthesia and Perioperative Care, University of California, San Francisco

In this article, primary lung endothelial cells were isolated and cultured from neonatal mice.