Institute of Molecular Genetics of the ASCR
2 ARTICLES PUBLISHED IN JoVE
Developmental Biology
Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
Polina Zjablovskaja *1,2, Petr Danek *1, Miroslava Kardosova 1, Meritxell Alberich-Jorda 1,2
1Department of Hemato-Oncology, Institute of Molecular Genetics of the ASCR, 2Childhood Leukaemia Investigation Prague, Department of Paediatric Haematology and Oncology, 2nd Faculty of Medicine, Charles University
Here detailed protocols for culturing the murine myeloid precursor 32D/G-CSF-R cell line, performing viral infections, and carrying out proliferation and differentiation assays are presented. This cell line is suitable for studying myeloid cell development, and the role of genes of interest in myeloid cell growth and neutrophilic differentiation.
Biology
Expression of Fluorescent Fusion Proteins in Murine Bone Marrow-derived Dendritic Cells and Macrophages
Jarmila Kralova 1,2, Daniela Glatzova 1,2,3, Simon Borna 1,2, Tomas Brdicka 1
1Laboratory of Leukocyte Signalling, Institute of Molecular Genetics of the ASCR, 2Faculty of Science, Charles University, 3Department of Biophysical Chemistry, J. Heyrovsky Institute of Physical Chemistry ASCR
In this article, we provide a detailed protocol for the expression of fluorescent fusion proteins in murine bone marrow derived dendritic cells and macrophages. The method is based on the transduction of bone marrow progenitors with retroviral constructs followed by differentiation into macrophages and dendritic cells in vitro.
