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Institute of Molecular Genetics of the ASCR

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Polina Zjablovskaja^*1,2, Petr Danek^*1, Miroslava Kardosova¹, Meritxell Alberich-Jorda^1,2

¹Department of Hemato-Oncology, Institute of Molecular Genetics of the ASCR, ²Childhood Leukaemia Investigation Prague, Department of Paediatric Haematology and Oncology, 2nd Faculty of Medicine, Charles University

Here detailed protocols for culturing the murine myeloid precursor 32D/G-CSF-R cell line, performing viral infections, and carrying out proliferation and differentiation assays are presented. This cell line is suitable for studying myeloid cell development, and the role of genes of interest in myeloid cell growth and neutrophilic differentiation.

Biology

Expression of Fluorescent Fusion Proteins in Murine Bone Marrow-derived Dendritic Cells and Macrophages

Jarmila Kralova^1,2, Daniela Glatzova^1,2,3, Simon Borna^1,2, Tomas Brdicka¹

¹Laboratory of Leukocyte Signalling, Institute of Molecular Genetics of the ASCR, ²Faculty of Science, Charles University, ³Department of Biophysical Chemistry, J. Heyrovsky Institute of Physical Chemistry ASCR

In this article, we provide a detailed protocol for the expression of fluorescent fusion proteins in murine bone marrow derived dendritic cells and macrophages. The method is based on the transduction of bone marrow progenitors with retroviral constructs followed by differentiation into macrophages and dendritic cells in vitro.