Zhejiang Academy of Traditional Chinese Medicine

4 ARTICLES PUBLISHED IN JoVE

Biology

A Protocol for Computer-Based Protein Structure and Function Prediction

Ambrish Roy^1,2, Dong Xu¹, Jonathan Poisson¹, Yang Zhang^1,2

¹Center for Computational Medicine and Bioinformatics, University of Michigan , ²Center for Bioinformatics and Department of Molecular Bioscience, University of Kansas

Guidelines for computer based structural and functional characterization of protein using the I-TASSER pipeline is described. Starting from query protein sequence, 3D models are generated using multiple threading alignments and iterative structural assembly simulations. Functional inferences are thereafter drawn based on matches to proteins with known structure and functions.

JoVE Core

Treatment with Vancomycin Loaded Calcium Sulphate and Autogenous Bone in an Improved Rabbit Model of Bone Infection

Yang Zhang^*1, Lifeng Shen^*2, Ping'er Wang³, Weixing Xi⁴, Zhongming Yu¹, Xiaowen Huang¹, Xuping Wang¹, Dan Shou¹

¹Department of Medicine, Zhejiang Academy of Traditional Chinese Medicine, ²Department of Orthopaedic Surgery, Tongde Hospital of Zhejiang Province, ³Institute of Orthopaedics and Traumatology, First Affiliated Hospital of Zhejiang Chinese Medical University, ⁴Department of Clinical Laboratory, Tongde Hospital of Zhejiang Province

This study presents an improved rabbit model infected with Staphylococcus aureus by blocking the same amount of bacteria in bone marrow. Vancomycin loaded calcium sulphate and autogenous bone are used for antibiotic and bone repair treatment. The protocol could be helpful for studying bone infection and regeneration.

Engineering

A Soft Tooling Process Chain for Injection Molding of a 3D Component with Micro Pillars

Yang Zhang¹, David Bue Pedersen¹, Michael Mischkot¹, Matteo Calaon¹, Federico Baruffi¹, Guido Tosello¹

¹Department of Mechanical Engineering, Technical University of Denmark

A protocol for fabricating injection molding inserts for complex geometry with micro features on surfaces employing Additive Manufacturing (AM) is presented.

Developmental Biology

Generation of a Mouse Artificial Decidualization Model with Ovariectomy for Endometrial Decidualization Research

Yang Zhang^*1,2,3, Zhe Zhang^*1,2,3, Nannan Kang^1,2,3, Xiaoqiang Sheng^1,2,3

¹Center for Reproductive Medicine and Obstetrics and Gynecology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, ²State Key Laboratory of Reproductive Medicine, Nanjing Medical University, ³Center for Molecular Reproductive Medicine, Nanjing University

Here, we describe the method of generating an artificial decidualization model using the ovariectomized mouse, a classic endometrial decidualization experiment in the research field of endometrial decidualization.