Nanfang Hospital, Southern Medical University
2 ARTICLES PUBLISHED IN JoVE
Bioengineering
Multiphoton Microscopic Observation of Vessels in Mouse Liver Tissue
Wen Rongrong *5, Li Ru *1, He Sixiao 1, Wang Ziqing 1, Huang Junhao 1, Zhao Liying 2, Tian Zhihui 3,4, Ma Qiang 1
1Department of Biopharmaceutics, School of Laboratory Medicine and Biotechnology, Southern Medical University, 2Department of General Surgery, Nanfang Hospital, Southern Medical University, 3Department of Stomatology, Nanfang Hospital, Southern Medical University, 4College of Stomatology, Southern Medical University, 5School of Laboratory Medicine and Biotechnology, Southern Medical University
In this experiment, a mouse is injected in its tail vein with Rhodamine B isothiocyanate–dextran that can stain blood vessels. After the liver is exposed and fixed, a specific part of the liver can be selected to observe the deep tissue in the living body using multiphoton microscopy.
Medicine
Multi-Gene Single Nucleotide Polymorphism Detection in Gastric Cancer Based on Ion Semiconductor Sequencing Platform
Kaihua Huang 1, Xiao Yan 1, Zhicheng Huang 1, Huayuan Liang 1, Zhiwei Li 1, Minghao Wang 1, Yong Wan 2, Suihai Wang 3,4, Liying Zhao 1
1Nanfang Hospital, Southern Medical University, 2Research and Development Department, Guangzhou Darui Biotechnology Co., Ltd, 3Key Laboratory of Antibody Engineering of Guangdong Higher Education Institutes, School of Laboratory Medicine and Biotechnology, Southern Medical University, 4Provincial Key Laboratory of Immune Regulation and Immunotherapy, Southern Medical University
This protocol proposes holistic laboratory procedures required to detect single nucleotide polymorphisms in gastric cancer samples based on an ion semiconductor sequencing platform. The target sequences, ligating adapters, library amplification and purification, and quality control criteria are also described in detail.
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