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Nanfang Hospital, Southern Medical University

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Multiphoton Microscopic Observation of Vessels in Mouse Liver Tissue

Wen Rongrong^*5, Li Ru^*1, He Sixiao¹, Wang Ziqing¹, Huang Junhao¹, Zhao Liying², Tian Zhihui^3,4, Ma Qiang¹

¹Department of Biopharmaceutics, School of Laboratory Medicine and Biotechnology, Southern Medical University, ²Department of General Surgery, Nanfang Hospital, Southern Medical University, ³Department of Stomatology, Nanfang Hospital, Southern Medical University, ⁴College of Stomatology, Southern Medical University, ⁵School of Laboratory Medicine and Biotechnology, Southern Medical University

In this experiment, a mouse is injected in its tail vein with Rhodamine B isothiocyanate–dextran that can stain blood vessels. After the liver is exposed and fixed, a specific part of the liver can be selected to observe the deep tissue in the living body using multiphoton microscopy.

Medicine

Multi-Gene Single Nucleotide Polymorphism Detection in Gastric Cancer Based on Ion Semiconductor Sequencing Platform

Kaihua Huang¹, Xiao Yan¹, Zhicheng Huang¹, Huayuan Liang¹, Zhiwei Li¹, Minghao Wang¹, Yong Wan², Suihai Wang^3,4, Liying Zhao¹

¹Nanfang Hospital, Southern Medical University, ²Research and Development Department, Guangzhou Darui Biotechnology Co., Ltd, ³Key Laboratory of Antibody Engineering of Guangdong Higher Education Institutes, School of Laboratory Medicine and Biotechnology, Southern Medical University, ⁴Provincial Key Laboratory of Immune Regulation and Immunotherapy, Southern Medical University

This protocol proposes holistic laboratory procedures required to detect single nucleotide polymorphisms in gastric cancer samples based on an ion semiconductor sequencing platform. The target sequences, ligating adapters, library amplification and purification, and quality control criteria are also described in detail.