Vilnius University
2 ARTICLES PUBLISHED IN JoVE
Biology
Chromatographic Purification of Highly Active Yeast Ribosomes
Arturas Meskauskas 1,2, Jonathan A. Leshin 1, Jonathan D. Dinman 1
1Department of Cell Biology and Molecular Genetics, University of Maryland , 2Department of Biotechnology and Microbiology, Vilnius University
Contamination of preparations of eukaryotic ribosomes purified by traditional methods by co-purifying nucleases and proteases negatively impacts on downstream biochemical and structural analyses. A rapid and simple chromatographic purification method is used to solve this problem using yeast ribosomes as a model system.
Biochemistry
Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy
Francesco Simone Ruggeri 1, Tomas Šneideris 1,2, Sean Chia 1, Michele Vendruscolo 1, Tuomas P. J. Knowles 1,3
1Centre for Misfolding Diseases, Department of Chemistry, University of Cambridge, 2Institute of Biotechnology, Life Sciences Center, Vilnius University, 3Cavendish Laboratory, Department of Physics, University of Cambridge
We describe the application of infrared nanospectroscopy and high-resolution atomic force microscopy to visualize the process of protein self-assembly into oligomeric aggregates and amyloid fibrils, which is closely associated with the onset and development of a wide range of human neurodegenerative disorders.
