Accedi

Vilnius University

2 ARTICLES PUBLISHED IN JoVE

Biology

Chromatographic Purification of Highly Active Yeast Ribosomes

Arturas Meskauskas^1,2, Jonathan A. Leshin¹, Jonathan D. Dinman¹

¹Department of Cell Biology and Molecular Genetics, University of Maryland , ²Department of Biotechnology and Microbiology, Vilnius University

Contamination of preparations of eukaryotic ribosomes purified by traditional methods by co-purifying nucleases and proteases negatively impacts on downstream biochemical and structural analyses. A rapid and simple chromatographic purification method is used to solve this problem using yeast ribosomes as a model system.

Biochemistry

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy

Francesco Simone Ruggeri¹, Tomas Šneideris^1,2, Sean Chia¹, Michele Vendruscolo¹, Tuomas P. J. Knowles^1,3

¹Centre for Misfolding Diseases, Department of Chemistry, University of Cambridge, ²Institute of Biotechnology, Life Sciences Center, Vilnius University, ³Cavendish Laboratory, Department of Physics, University of Cambridge

We describe the application of infrared nanospectroscopy and high-resolution atomic force microscopy to visualize the process of protein self-assembly into oligomeric aggregates and amyloid fibrils, which is closely associated with the onset and development of a wide range of human neurodegenerative disorders.