Wuyi University
2 ARTICLES PUBLISHED IN JoVE
Biochemistry
Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation
Manabu Niimi 1, Haizhao Yan 1, Yajie Chen 1, Yao Wang 2, Jianglin Fan 1,2
1Department of Molecular Pathology, Interdisciplinary Graduate School of Medicine, University of Yamanashi, 2School of Biotechnology and Health Sciences, Wuyi University
Several methods have been used for analyzing plasma lipoproteins; however, ultracentrifugation is still one of the most popular and reliable methods. Here, we describe a method regarding how to isolate lipoproteins from plasma using sequential density ultracentrifugation and how to analyze the apolipoproteins for both diagnostic and research purposes.
Bioengineering
Facilitating Cerebral Organoid Culture via Lateral Soft Light Illumination
Bangzhu Chen 1,2, Qinrong Lin 1,2, Nengqing Liu 1,2, Diyu Chen 1,2, Yinghui Zhang 3, Xiaofang Sun 1,2
1Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, The Third Affiliated Hospital of Guangzhou Medical University, 2Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, 3School of Biotechnology and Health Sciences, Wuyi University
Cerebral organoids provide unprecedented opportunities for studying organ development and human disease pathology. Although great success has been achieved with cerebral organoid culture systems, there are still operational difficulties in applying this technology. The present protocol describes a cerebral organoid procedure that facilitates medium change and organoid transfer.
