Accedi

Wuyi University

2 ARTICLES PUBLISHED IN JoVE

Biochemistry

Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation

Manabu Niimi¹, Haizhao Yan¹, Yajie Chen¹, Yao Wang², Jianglin Fan^1,2

¹Department of Molecular Pathology, Interdisciplinary Graduate School of Medicine, University of Yamanashi, ²School of Biotechnology and Health Sciences, Wuyi University

Several methods have been used for analyzing plasma lipoproteins; however, ultracentrifugation is still one of the most popular and reliable methods. Here, we describe a method regarding how to isolate lipoproteins from plasma using sequential density ultracentrifugation and how to analyze the apolipoproteins for both diagnostic and research purposes.

Bioengineering

Facilitating Cerebral Organoid Culture via Lateral Soft Light Illumination

Bangzhu Chen^1,2, Qinrong Lin^1,2, Nengqing Liu^1,2, Diyu Chen^1,2, Yinghui Zhang³, Xiaofang Sun^1,2

¹Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, The Third Affiliated Hospital of Guangzhou Medical University, ²Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, ³School of Biotechnology and Health Sciences, Wuyi University

Cerebral organoids provide unprecedented opportunities for studying organ development and human disease pathology. Although great success has been achieved with cerebral organoid culture systems, there are still operational difficulties in applying this technology. The present protocol describes a cerebral organoid procedure that facilitates medium change and organoid transfer.