2.7K Views
•
10:47 min
•
May 5th, 2023
DOI :
May 5th, 2023
•0:00
Introduction
1:21
Preparation of siRNA Mixture for Mitochondrial DNA Synthesis
2:00
Cell Transfection and 5‐Bromo‐2’‐Deoxyuridine (BrdU) Incorporation in HeLa Cells
3:14
5-Bromo-2'-Deoxyuridine (BrdU) Labeling of Mitochondrial DNA in HeLa Cells
4:51
HeLa Cell Fixation with Hoechst 33342 and Blocking
5:51
Addition of Primary and Secondary Antibodies to the Transfected HeLa Cells to Monitor the mtDNA Distribution
7:49
Quantitative Image Analysis to Measure the mtDNA and Gene Expression Levels in the Transfected HeLa Cells
相关视频
12.9K Views
8.5K Views
8.0K Views
8.1K Views
7.1K Views
8.6K Views
7.2K Views
9.0K Views
4.1K Views
4.0K Views
版权所属 © 2025 MyJoVE 公司版权所有,本公司不涉及任何医疗业务和医疗服务。