CHU Nantes

3 ARTICLES PUBLISHED IN JoVE

Medicine

Techniques of Sleeve Gastrectomy and Modified Roux-en-Y Gastric Bypass in Mice

Audrey Ayer¹, Frederic Borel¹, Francois Moreau¹, Xavier Prieur¹, Michel Neunlist², Bertrand Cariou³, Claire Blanchard^*1,4, Cedric Le May^*1

¹l'Institut du Thorax, INSERM, CNRS, UNIV Nantes, ²CHU Nantes, Institut des Maladies de l'Appareil Digestif, INSERM U913, ³l'Institut du Thorax, INSERM, CNRS, UNIV Nantes, CHU Nantes, ⁴Service de Clinique Chirurgicale Digestive et Endocrinienne, CHU de Nantes

Bariatric surgery is the most efficient way to reduce body weight and the deadly metabolic complications (diabetes, obesity, and dyslipidemia) frequently associated with morbid obesity. Mouse models of bariatric surgery represent a unique asset for deciphering molecular mechanisms behind the beneficial effects of these surgeries on diabetes, hypertension, and dyslipidemia.

Immunology and Infection

In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients

Séverine Bézie^1,2, Claire Usal^1,2, Carole Guillonneau^1,2

¹Centre de Recherche en Transplantation et Immunologie UMR 1064, INSERM, Université de Nantes, ²Institut de Transplantation Urologie Néphrologie (ITUN), CHU Nantes

Here, we present a protocol to induce tolerance in transplantation, and assess in vitro and in vivo the suppressive capacity of distinct cell subsets from the recipient and the immune status of the recipient toward donor or exogenous antigens.

Medicine

Single-Anastomosis Duodeno-Ileal Bypass with Sleeve Gastrectomy Model in Mice

Samuel Frey^1,2, Audrey Ayer¹, Thibaud Sotin¹, Victoria Lorant¹, Bertrand Cariou¹, Claire Blanchard^*1,2, Cédric Le May^*1

¹CHU Nantes, CNRS, INSERM, l'institut du thorax, Nantes Université, ²Chirurgie Cancérologique, Digestive et Endocrinienne, Institut des Maladies de l'Appareil Digestif, Hôtel Dieu, CHU Nantes

Single-anastomosis duodeno-ileal bypass (SADI-S) is an emerging bariatric procedure with important metabolic effects. In this article, we present a reliable and reproducible model of SADI-S in mice.