The Affiliated Zhangjiagang Hospital of Soochow University

5 ARTICLES PUBLISHED IN JoVE

Neuroscience

Promotion of Survival and Differentiation of Neural Stem Cells with Fibrin and Growth Factor Cocktails after Severe Spinal Cord Injury

Paul Lu^1,2, Lori Graham², Yaozhi Wang², Di Wu², Mark Tuszynski^1,2

¹Veterans Administration Medical Center, San Diego, ²Department of Neurosciences, University of California, San Diego

Fibrin matrices containing growth factors were used to retain grafted neural stem cells into sites of complete spinal cord transection. Grafted cells completely filled the lesion cavity and differentiated into multiple neural cell types, including neurons that extended axons into host spinal cord over long distances.

JoVE Journal

Thermocapillary Convection Space Experiment on the SJ-10 Recoverable Satellite

Li Duan^*1,2, Yongli Yin^*3, Jia Wang^*1, Qi Kang^1,2, Di Wu¹, Huan Jiang¹, Pu Zhang¹, Liang Hu¹

¹National Microgravity Laboratory, Institute of Mechanics, Chinese Academy of Sciences, ²School of Engineering Sciences, University of Chinese Academy of Sciences, ³China Astronaut Research and Training Center

A protocol for the space payload design, the space experiment on thermocapillary convection, and analyses of experimental data and images are presented in this paper.

JoVE Journal

Rapid Determination of Antibody-Antigen Affinity by Mass Photometry

Di Wu¹, Grzegorz Piszczek¹

¹Biophysics Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health

We describe a single-molecule approach to antigen-antibody affinity measurements using mass photometry (MP). The MP-based protocol is fast, accurate, uses a very small amount of material, and does not require protein modification.

Behavior

An Assessment Method and Toolkit to Evaluate Keyboard Design on Smartphones

Yincheng Wang¹, Ke Wang¹, Yuqi Huang¹, Di Wu², Jian Wu³, Jibo He¹

¹Department of Psychology, School of Social Sciences, Tsinghua University, ²Department of Computer Science, Beijing Normal University, ³Haier Innovation Design Center, Haier Company

The presented protocol integrates various evaluation methods and demonstrates a method to evaluate the keyboard design on smartphones. Pairs matched by English characters are proposed as the input material, and the transition time between two keys is used as the dependent variable.

Biochemistry

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Di Wu¹, Mengfan He¹, Dawei Li¹

¹Center for Translational Medicine, The Affiliated Zhangjiagang Hospital of Soochow University

The protocol describes a step-by-step method to purify ubiquitinated proteins from mammalian cells using the p53 tumor suppressor protein as an example. Ubiquitinated p53 proteins were purified from cells under stringent nondenaturing and denaturing conditions.