University of South Dakota
2 ARTICLES PUBLISHED IN JoVE
Behavior
Modeling Fetal Alcohol Spectrum Disorders in Zebrafish to Characterize the Impact of an Adverse Embryonic Environment on Adult Social Behavior
Mindy Rampersad 1, Yohaan Fernandes 1
1Department of Biology, University of South Dakota
The goal of the current protocol is to outline the steps necessary to establish and use a social preference assay for adult zebrafish and demonstrate that it can be used to characterize ethanol-induced social defects.
Biochemistry
Measuring Enzymatic Activity of Neurodevelopmental Disorder-Associated Deubiquitylating Enzymes via an In Vitro Ubiquitin Chain Cleavage Assay
Intisar Koch 1, Matthew J. Schellenberg 2, Francisco Bustos 1,3
1Pediatrics and Rare Diseases Group, Sanford Research, 2Department of Biochemistry and Molecular Biology, Mayo Clinic, 3Department of Pediatrics, Sanford School of Medicine, University of South Dakota
This protocol explains how to measure the effect of genetic variation associated with neurodevelopmental disorders on deubiquitylating enzyme activity by combining recombinant protein purification with ubiquitin chain cleavage assays.
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