University of South Dakota

2 ARTICLES PUBLISHED IN JoVE

Behavior

Modeling Fetal Alcohol Spectrum Disorders in Zebrafish to Characterize the Impact of an Adverse Embryonic Environment on Adult Social Behavior

Mindy Rampersad¹, Yohaan Fernandes¹

¹Department of Biology, University of South Dakota

The goal of the current protocol is to outline the steps necessary to establish and use a social preference assay for adult zebrafish and demonstrate that it can be used to characterize ethanol-induced social defects.

Biochemistry

Measuring Enzymatic Activity of Neurodevelopmental Disorder-Associated Deubiquitylating Enzymes via an In Vitro Ubiquitin Chain Cleavage Assay

Intisar Koch¹, Matthew J. Schellenberg², Francisco Bustos^1,3

¹Pediatrics and Rare Diseases Group, Sanford Research, ²Department of Biochemistry and Molecular Biology, Mayo Clinic, ³Department of Pediatrics, Sanford School of Medicine, University of South Dakota

This protocol explains how to measure the effect of genetic variation associated with neurodevelopmental disorders on deubiquitylating enzyme activity by combining recombinant protein purification with ubiquitin chain cleavage assays.