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Universitè de Montrèal

5 ARTICLES PUBLISHED IN JoVE

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Biology

Dissecting the Non-human Primate Brain in Stereotaxic Space
Mark W. Burke 1, Shahin Zangenehpour 2, Denis Boire 3, Maurice Ptito 2
1Department of Physiology, University of Montreal, 2School of Optometry, University of Montreal, 3Département de chimie-biologie
, Université du Québes à Trois-Rivières

The non-human primate is an important translational species for our understanding of the normal processing of the brain. The anatomical organization of the primate brain can provide important insights into normal and pathological conditions in humans.

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Biology

Brain Banking: Making the Most of your Research Specimens
Mark W. Burke 1, Shahin Zangenehpour 2, Maurice Ptito 2
1Department of Physiology, University of Montreal, 2School of Optometry, University of Montreal

Brain banking and systematic sampling of biological material provides the basis for unbiased stereology and maximizes the potential data obtained from each specimen.

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Biology

The Gateway to the Brain: Dissecting the Primate Eye
Mark Burke 1, Shahin Zangenehpour 2, Joseph Bouskila 2, Denis Boire 3, Maurice Ptito 2
1Department of Physiology, University of Montreal, 2School of Optometry, University of Montreal, 3Departement de chimie-biologie, Universite du Quebec a Trois-Rivieres

The non-human primate is an important translational species for our understanding of development and aging. The anatomical organization of the primate retina may provide important insights into normal and pathological conditions in humans.

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Biology

Knowing What Counts: Unbiased Stereology in the Non-human Primate Brain
Mark Burke 1, Shahin Zangenehpour 2, Peter R. Mouton 3, Maurice Ptito 2
1Department of Physiology, University of Montreal, 2Ecole d’optometrie, University of Montreal, 3Stereology Resource Center

The anatomical organization of the primate brain can provide important insights into normal and pathological conditions in humans. Unbiased stereology is a method for accurately and efficiently estimating the total neuron number (or other cell type) in a given reference space1.

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Biology

Batch Immunostaining for Large-Scale Protein Detection in the Whole Monkey Brain
Shahin Zangenehpour 1,2, Mark W. Burke 2, Avi Chaudhuri 3, Maurice Ptito 2
1Cognitive Neuroscience Unit, Montreal Neurological Institute, 2Ècole d’Optomètrie, Universitè de Montrèal, 3Department of Psychology, McGill University

Large-scale immunodetection of target proteins across the entire primate brain is possible by employing novel tissue embedding and sectioning methods combined with the use of creative apparatus for batch staining of multiple free-floating sections at a given time.

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