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Universitè de Montrèal

5 ARTICLES PUBLISHED IN JoVE

Biology

Dissecting the Non-human Primate Brain in Stereotaxic Space

Mark W. Burke¹, Shahin Zangenehpour², Denis Boire³, Maurice Ptito²

¹Department of Physiology, University of Montreal, ²School of Optometry, University of Montreal, ³Département de chimie-biologie , Université du Québes à Trois-Rivières

The non-human primate is an important translational species for our understanding of the normal processing of the brain. The anatomical organization of the primate brain can provide important insights into normal and pathological conditions in humans.

Biology

Brain Banking: Making the Most of your Research Specimens

Mark W. Burke¹, Shahin Zangenehpour², Maurice Ptito²

¹Department of Physiology, University of Montreal, ²School of Optometry, University of Montreal

Brain banking and systematic sampling of biological material provides the basis for unbiased stereology and maximizes the potential data obtained from each specimen.

Biology

The Gateway to the Brain: Dissecting the Primate Eye

Mark Burke¹, Shahin Zangenehpour², Joseph Bouskila², Denis Boire³, Maurice Ptito²

¹Department of Physiology, University of Montreal, ²School of Optometry, University of Montreal, ³Departement de chimie-biologie, Universite du Quebec a Trois-Rivieres

The non-human primate is an important translational species for our understanding of development and aging. The anatomical organization of the primate retina may provide important insights into normal and pathological conditions in humans.

Biology

Knowing What Counts: Unbiased Stereology in the Non-human Primate Brain

Mark Burke¹, Shahin Zangenehpour², Peter R. Mouton³, Maurice Ptito²

¹Department of Physiology, University of Montreal, ²Ecole d’optometrie, University of Montreal, ³Stereology Resource Center

The anatomical organization of the primate brain can provide important insights into normal and pathological conditions in humans. Unbiased stereology is a method for accurately and efficiently estimating the total neuron number (or other cell type) in a given reference space¹.

Biology

Batch Immunostaining for Large-Scale Protein Detection in the Whole Monkey Brain

Shahin Zangenehpour^1,2, Mark W. Burke², Avi Chaudhuri³, Maurice Ptito²

¹Cognitive Neuroscience Unit, Montreal Neurological Institute, ²Ècole d’Optomètrie, Universitè de Montrèal, ³Department of Psychology, McGill University

Large-scale immunodetection of target proteins across the entire primate brain is possible by employing novel tissue embedding and sectioning methods combined with the use of creative apparatus for batch staining of multiple free-floating sections at a given time.