Cell-based Flow Cytometry Assay to Measure Cytotoxic ActivityAlessandra Noto 1, Pearline Ngauv 1, Lydie Trautmann 1
1Vaccine and Gene Therapy Institute of Florida
This protocol describes a sensitive, cell-based cytotoxicity assay. By enumerating the decrease in frequency of live target CD4+ T cells in the presence of an increasing number of effector CD8+ T cells, this assay allows for the direct assessment of cytolytic activity of antigen-specific CD8+ T cells.
