Accedi

Vaccine and Gene Therapy Institute of Florida

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity

Alessandra Noto¹, Pearline Ngauv¹, Lydie Trautmann¹

¹Vaccine and Gene Therapy Institute of Florida

This protocol describes a sensitive, cell-based cytotoxicity assay. By enumerating the decrease in frequency of live target CD4+ T cells in the presence of an increasing number of effector CD8+ T cells, this assay allows for the direct assessment of cytolytic activity of antigen-specific CD8+ T cells.