Vaccine and Gene Therapy Institute of Florida

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Immunology and Infection

Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity

Alessandra Noto¹, Pearline Ngauv¹, Lydie Trautmann¹

¹Vaccine and Gene Therapy Institute of Florida

This protocol describes a sensitive, cell-based cytotoxicity assay. By enumerating the decrease in frequency of live target CD4+ T cells in the presence of an increasing number of effector CD8+ T cells, this assay allows for the direct assessment of cytolytic activity of antigen-specific CD8+ T cells.