The University of Adelaide
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface
Marcia Arenas-Hernandez 1, Elly N. Sanchez-Rodriguez 1, Tara N. Mial 1, Sarah A. Robertson 2, Nardhy Gomez-Lopez 1,3,4
1Department of Obstetrics & Gynecology, Wayne State University School of Medicine, 2School of Paediatrics and Reproductive Health, Research Centre for Reproductive Health, the Robinson Research Institute, The University of Adelaide, 3Department of Immunology & Microbiology, Wayne State University School of Medicine, 4Perinatology Research Branch, NICHD/NIH/DHHS
Described herein is a protocol to isolate and analyze the infiltrating leukocytes of tissues at the maternal-fetal interface (uterus, decidua, and placenta) of mice. This protocol maintains the integrity of most cell surface markers and yields enough viable cells for downstream applications including flow cytometry analysis.
Biochemistry
Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening
Jia Quyen Truong *1, Stephanie Nguyen *1,2, John B. Bruning 1,2, Keith E. Shearwin 1
1School of Biological Sciences, The University of Adelaide, 2Institute of Photonics and Advanced Sensing (IPAS), School of Biological Sciences, The University of Adelaide
This article presents a method to generate protein crystals derivatized with I3C (5-amino-2,4,6-triiodoisophthalic acid) using microseeding to generate new crystallization conditions in sparse matrix screens. The trays can be set up using liquid dispensing robots or by hand.
