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The University of Adelaide

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface

Marcia Arenas-Hernandez¹, Elly N. Sanchez-Rodriguez¹, Tara N. Mial¹, Sarah A. Robertson², Nardhy Gomez-Lopez^1,3,4

¹Department of Obstetrics & Gynecology, Wayne State University School of Medicine, ²School of Paediatrics and Reproductive Health, Research Centre for Reproductive Health, the Robinson Research Institute, The University of Adelaide, ³Department of Immunology & Microbiology, Wayne State University School of Medicine, ⁴Perinatology Research Branch, NICHD/NIH/DHHS

Described herein is a protocol to isolate and analyze the infiltrating leukocytes of tissues at the maternal-fetal interface (uterus, decidua, and placenta) of mice. This protocol maintains the integrity of most cell surface markers and yields enough viable cells for downstream applications including flow cytometry analysis.

Biochemistry

Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening

Jia Quyen Truong^*1, Stephanie Nguyen^*1,2, John B. Bruning^1,2, Keith E. Shearwin¹

¹School of Biological Sciences, The University of Adelaide, ²Institute of Photonics and Advanced Sensing (IPAS), School of Biological Sciences, The University of Adelaide

This article presents a method to generate protein crystals derivatized with I3C (5-amino-2,4,6-triiodoisophthalic acid) using microseeding to generate new crystallization conditions in sparse matrix screens. The trays can be set up using liquid dispensing robots or by hand.

Biology

Isolation of Chondrocytes and Chondroprogenitors using Fibronectin Adhesion and Migratory Assay

Elizabeth Vinod^1,2, Ganesh Parasuraman², Abel Livingston³, Solomon Sathishkumar¹, Boopalan Ramasamy^4,5

¹Department of Physiology, Christian Medical College, ²Centre for Stem Cell Research (A unit of InStem, Bengaluru), Christian Medical College, ³Department of Orthopedics, Christian Medical College, ⁴Faculty of Health and Medical Sciences, The University of Adelaide, ⁵Department of Orthopedics and Trauma, Royal Adelaide Hospital

This protocol details the isolation of chondrocytes, Fibronectin Adhesion Assay-derived Chondroprogenitors (FAA-CPs), and Migratory Chondroprogenitors (MCPs) from human articular cartilage. It covers enzymatic digestion, fibronectin adhesion, and migration-based assays for isolating and characterizing these cells.