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Universidad de la República (UDELAR)

3 ARTICLES PUBLISHED IN JoVE

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Cancer Research

How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases
Mercedes Rodriguez-Teja 1, Claudia Breit 2, Mitchell Clarke 3, Kamil Talar 3, Kai Wang 3, Mohammad A. Mohammad 3, Sage Pickwell 3, Guillermina Etchandy 1, Graeme J. Stasiuk 3, Justin Sturge 3
1Departamento de Genética, Facultad de Medicina, Universidad de la República (UDELAR), 2Department of Mechanistic Cell Biology, Max Planck Institute of Molecular Physiology, 3School of Biological, Biomedical & Environmental Sciences, University of Hull

Here we explain a protocol for modelling the biophysical microenvironment where crosslinking and increased stiffness of the basement membrane (BM) induced by advanced glycation endproducts (AGEs) has pathological relevance.

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Developmental Biology

Atomic Force Microscopy to Study the Physical Properties of Epidermal Cells of Live Arabidopsis Roots
Ines Rauschert *2, Juan Claudio Benech *2, Martha Sainz 1, Omar Borsani 1, Mariana Sotelo-Silveira 1
1Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía, Universidad de la República (UdelaR), 2Laboratorio de Señalización Celular y Nanobiología, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE)

The atomic force microscopy indentation protocol offers the possibility to dissect the role of the physical properties of the cell wall of a particular cell of a tissue or organ during normal or constrained growth (i.e., under water deficit).

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Developmental Biology

Polysome Purification from Soybean Symbiotic Nodules
María Martha Sainz 1, Carla Valeria Filippi 1, Guillermo Eastman 2,3, Mariana Sotelo-Silveira 1, C. Mauro Martinez 1, Omar Borsani 1, José Sotelo-Silveira 2,4
1Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía, Universidad de la República, 2Departamento de Genómica, Instituto de Investigaciones Biológicas Clemente Estable, 3Department of Biology, University of Virginia, 4Departamento de Biología Celular y Molecular, Facultad de Ciencias, Universidad de la República

This protocol describes a method for eukaryotic polysome purification from intact soybean nodules. After sequencing, standard pipelines for gene expression analysis can be used to identify differentially expressed genes at the transcriptome and translatome levels.

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