Universita’ di Pisa

2 ARTICLES PUBLISHED IN JoVE

Cancer Research

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Rocco Sciarrillo^1,2,3, Anna Wojtuszkiewicz¹, Irsan E. Kooi⁴, Valentina E. Gómez³, Ugo Boggi⁵, Gerrit Jansen⁶, Gert-Jan Kaspers^1,7, Jacqueline Cloos^*1, Elisa Giovannetti^*3,8,9

¹Department of Pediatric Oncology/Hematology, VU University Medical Center, ²Department of Hematology, VU University Medical Center, ³Department of Medical Oncology, VU University Medical Center, ⁴Department of Clinical Genetics, VU University Medical Center, ⁵Division of General and Transplant Surgery, Azienda Ospedaliera Universitaria Pisana, Universita’ di Pisa, ⁶Amsterdam Immunology and Rheumatology Center, VU University Medical Center, ⁷Princess Máxima Center for Pediatric Oncology, ⁸Cancer Pharmacology Lab, AIRC Start-Up Unit, University of Pisa, ⁹Institute of Nanoscience and Nanotechnology, CNR-Nano

Here we describe a protocol aimed at investigating the impact of aberrant splicing on drug resistance in solid tumors and hematological malignancies. To this goal, we analyzed the transcriptomic profiles of parental and resistant in vitro models through RNA-seq and established a qRT-PCR based method to validate candidate genes.

Medicine

Robot-Assisted Radical Antegrade Modular Pancreatosplenectomy Including Resection and Reconstruction of the Spleno-Mesenteric Junction

Niccolò Napoli¹, Emanuele F. Kauffmann¹, Francesca Menonna¹, Sara Iacopi¹, Concetta Cacace¹, Ugo Boggi¹

¹Division of General and Transplant Surgery, University of Pisa

The robotic technique shown herein aims at faithfully reproducing the open procedure for radical treatment of cancer of the body-tail of the pancreas. The protocol also demonstrates the ability to master involvement of major peripancreatic vessels without conversion to open surgery.