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Universita’ di Pisa

2 ARTICLES PUBLISHED IN JoVE

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Cancer Research

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
Rocco Sciarrillo 1,2,3, Anna Wojtuszkiewicz 1, Irsan E. Kooi 4, Valentina E. Gómez 3, Ugo Boggi 5, Gerrit Jansen 6, Gert-Jan Kaspers 1,7, Jacqueline Cloos *1, Elisa Giovannetti *3,8,9
1Department of Pediatric Oncology/Hematology, VU University Medical Center, 2Department of Hematology, VU University Medical Center, 3Department of Medical Oncology, VU University Medical Center, 4Department of Clinical Genetics, VU University Medical Center, 5Division of General and Transplant Surgery, Azienda Ospedaliera Universitaria Pisana, Universita’ di Pisa, 6Amsterdam Immunology and Rheumatology Center, VU University Medical Center, 7Princess Máxima Center for Pediatric Oncology, 8Cancer Pharmacology Lab, AIRC Start-Up Unit, University of Pisa, 9Institute of Nanoscience and Nanotechnology, CNR-Nano

Here we describe a protocol aimed at investigating the impact of aberrant splicing on drug resistance in solid tumors and hematological malignancies. To this goal, we analyzed the transcriptomic profiles of parental and resistant in vitro models through RNA-seq and established a qRT-PCR based method to validate candidate genes.

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Medicine

Robot-Assisted Radical Antegrade Modular Pancreatosplenectomy Including Resection and Reconstruction of the Spleno-Mesenteric Junction
Niccolò Napoli 1, Emanuele F. Kauffmann 1, Francesca Menonna 1, Sara Iacopi 1, Concetta Cacace 1, Ugo Boggi 1
1Division of General and Transplant Surgery, University of Pisa

The robotic technique shown herein aims at faithfully reproducing the open procedure for radical treatment of cancer of the body-tail of the pancreas. The protocol also demonstrates the ability to master involvement of major peripancreatic vessels without conversion to open surgery.

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